Abstracts (poster) - Wissenschaft Online
Abstracts (poster) - Wissenschaft Online
Abstracts (poster) - Wissenschaft Online
Create successful ePaper yourself
Turn your PDF publications into a flip-book with our unique Google optimized e-Paper software.
Rafal Archacki, T.J. Sarnowski, J. Halibart-Puzio, D. Buszewicz, M. Prymakowska-<br />
Bosak, M. Kuras, C. Koncz, A. Jerzmanowski<br />
BRM bromodomain-ATPase and ATSWI3C, representing<br />
putative subunits of SWI/SNF chromatin remodeling<br />
complexes, control similar developmental functions in<br />
Arabidopsis<br />
Among the factors that serve to modify chromatin structure, SWI/SNF chromatin<br />
remodeling complexes define conserved and well-characterized group. However, no<br />
SWI/SNF complex has been purified and characterized in higher plants so far, yet its<br />
existence is highly probable. Four genes encoding homologues of Swi2/Snf2 ATPase<br />
(BRM, SYD, CHR12 and CHR23) and four encoding homologues of Swi3 subunit<br />
(ATSWI3A, ATSWI3B, ATSWI3C and ATSWI3D), as well as a single Snf5 orthologue<br />
(BSH) have been identified in Arabidopsis (1). This makes a number of possibilities for<br />
assembly of plant SWI/SNF complexes. In the lack of structural and biochemical data,<br />
homology analyses and interpretation of genetic and in vitro interactions are the best<br />
tools for investigating SWI/SNF complex composition and function.<br />
Here we show a comparative analysis of brm and atswi3c null mutants. Both of them<br />
display similar (but not identical) developmental alterations, including semidwarfism,<br />
leaf curling, inhibition of root elongation, homeotic-like changes in flowers, and defects<br />
in pollen development. These observations, together with the results showing that BRM<br />
and SWI3C interact in yeast two-hybrid assay (2), suggest that BRM and SWI3C proteins<br />
exist in the same SWI/SNF chromatin remodeling complex. Our analyses of brm atswi3c<br />
double mutants further support this hypothesis, as the brm atswi3c plants display brm<br />
phenotype. Nonetheless, certain differences between phenotypic traits of atswi3c and<br />
brm mutants, such as complete sterility of brm and the occurrence of unfused carpels in<br />
brm flowers, indicate that the biological functions of these two SWI/SNF subunits are not<br />
completely overlapping.<br />
Literature<br />
(1) Jerzmanowski A. SWI/SNF chromatin remodeling and linker histones in plants.<br />
Biochim Biophys Acta. 2007 May-Jun;1769(5-6):330-45<br />
(2) Farrona S, Hurtado L, Bowman JL, Reyes JC. The Arabidopsis thaliana SNF2 homolog<br />
AtBRM controls shoot development and flowering. Development. 2004 Oct;131(20):4965-<br />
75<br />
contact:<br />
M.Sc Rafal Archacki<br />
University of Warsaw<br />
Laboratory of Plant Molecular Biology<br />
rafa@ibb.waw.pl<br />
Pawinskiego 5A<br />
02-106 Warsaw (Poland)<br />
additional information<br />
Affiliation of T.J. Sarnowski, J. Halibart-Puzio, and D. Buszewicz: Polish Academy of Sciences,<br />
Institute of Biochemistry and Biophysics, Pawinskiego 5A, 02-106 Warsaw, Poland<br />
Second affiliation of M. Prymakowska-Bosak and A. Jerzmanowski: Polish Academy of Sciences,<br />
Institute of Biochemistry and Biophysics, Pawinskiego 5A, 02-106 Warsaw, Poland<br />
Affiliation of M. Kuras: University of Warsaw, Department of Ecotoxicology, Miecznikowa 1, 02-096<br />
Warsaw, Poland<br />
Affiliation of C. Koncz: Max-Planck Institut für Züchtungsforschung, Carl-von-Linné-Weg 10, D-<br />
50829 Köln, Germany
Change language