Abstracts (poster) - Wissenschaft Online

Devi Thiagarajan, Sanjeev Khosla
Functional characterisation of mDnmt2
Inspite of having all the signature motifs for a DNA methyl transferase, Dnmt2 seems to
have a weak DNA methyl transferase activity. It has also been shown to have a tRNA
methyltransferase activity whose significance is not clear. The purpose of this study was
to probe the function of the mouse Dnmt2 protein through its interacting partners. We
have identified a few interacting partners of Dnmt2 through Immunoprecipitation and
GST pull down assays. Most of the interacting partners were found to be involved in RNA
metabolism. These interactions have been confirmed through mammalian two hybrid
and reverse Co-Immunoprecipitation experiments. The significance of Dnmt2 interacting
partners, in light of our experiments examining the subcellular localization of Dnmt2 and
its various deletion mutations would be presented in correlation to Dnmt2 function.
contact:
Senior research fellow Devi Thiagarajan
Centre for DNA Fingerprinting and Diagnostics
Centre for DNA Fingerprinting and Diagnostics
devi@cdfd.org.in
ECIL Road, Nacharam
500076 Hyderabad (India)