Abstracts (poster) - Wissenschaft Online

More documents

Recommendations

Info

Annelen Schemm, Sabine Neumann, Pamela Strissel, Cord-Michael Becker Role of NRSF/ hREST4 in Neuronalisation of Tumors The neuron-restrictive silencer factor (NRSF) represses transcription of neuronal genes in non-neuronal tissues by binding to the neuron-restrictive silencer DNA element (NRSE). The expression of neuronal cell markers in malignant tumors, e.g. Small Cell Lung Cancer (SCLC), frequently correlates with paraneoplastic disorders but also with a higher invasivness and a poorer prognosis (1,2,3). In a variety of cell lines, NRSF and other neuronal gene expression patterns were determined using RT- PCR. For some cell lines absence of the NRSF transcript correlated with a presence of neuronal gene expression, while its presence correlated with neuronal gene silencing. In some cell lines, however, neuronal transcription occurred despite the presence of an NRSF transcript. Interestingly the NRSF splice variant, hREST4, was detectable in those cell lines. hREST4 is known to have nuclear localisation , supporting a role in transcriptional regulation. To test for a possible activating function of hREST4, luciferase assays were carried out using cell lines with and without hREST4/ NRSF transcripts, upon transfection with plasmids containing the luciferase gene under control of the NRSE of GlyR alpha1. However, no clear hREST4 function could be determined using that multi- cell line approach in the presense of full length NRSF. But there was a perfect correlation of hREST4 and GlyR alpha1 transcript levels when hREST4 expression levels were enhanced via drug treatment within one cell line, supporting our hypothesis. The mechanism of NRSF loss and the possible transcriptional activity of hREST4 may contribute to the constitutive activation of neuronal genes leading to deregulated growth and altered invasion behavior. Literature (1) Gurrola-Diaz, C., Lacroix, J., Dihlmann, S., Becker, C.-M., und von Knebel Doeberitz, M. (2003): Reduced expression of the neuron restrictive silencer factor permits transcription of glycine receptor α1 subunit in small-cell lung cancer cells. Oncogene 22, 5636-5645. (2) S.B. Neumann, R. Seitz, A. Gorzella, A. Heister, M. von Knebel-Döberitz and C.-M. Becker (2004): Relaxation of glycine receptor and onconeural gene transcription in NRSFdeficient small cell lung cancer cell lines, Brain Res. Mol. Brain Res. 120, 173-181. (3) Butler, M. H., Hayashi, A., Ohkoshi, N., Villmann, C., Becker, C.-M., Feng, G., de Camilli, P., und Solimena, M. (2000) Autoimmunity against gephyrin in stiff man syndrome. Neuron 26, 307–312. contact: Annelen Schemm Universität Erlangen- Nürnberg Institut für Biochemie annelen.schemm@biochem.uni-erlangen.de Fahrsstrasse 17 91054 Erlangen (Deutschland)
Ernst Aichinger, Aleksandra Erilova, Grigory Makarevich, Claudia Köhler Role of the Mi-2 homolog PICKLE in repression of Polycomb group target genes in Arabidopsis Mi-2 subunits are part of the NuRD (Nucleosome remodeling and deacetylase) complex and are widely conserved within the animal and plant kingdom. Current models propose varying enzymatic functions in transcriptional repression as well as in derepression. PICKLE (PKL) is homologous to Mi-2 alpha subunits and is involved in the specification of different developmental fates. PKL restricts pluripotency by stable repression of embryonic traits after germination. Moreover, PKL is required throughout the plant life cycle and lack of PKL function causes activation of the Polycomb group (PcG) target gene PHERES1. PHERES1 expression is restricted to seed development and is actively repressed during vegetative development by the action of different Polycomb group complexes. Therefore, we addressed the question whether activation of Polycomb group target genes in pkl mutants is caused by a mechanistic interaction of PKL and Polycomb group complexes. contact: Ernst Aichinger ETH Zürich Institute of Plant Sciences aernst@ethz.ch Universitätsstrasse 2 8092 Zurich (Switzerland)
Page 1 and 2:
Abstracts (poster) Mark Wossidlo γ
Page 3 and 4:
Silke Götze, Sonja Sievers, Oliver
Page 5 and 6:
Gunter Reuter, Thomas Rudolph, Sand
Page 7 and 8:
The function of two conserved eleme
Page 9 and 10:
Bernd Schmeck, Janina Lorenz, Phili
Page 11 and 12:
Anna Katharina Sedello, Gabriele Pu
Page 13 and 14:
Maja Klug, Sven Heinz, Lucia Schwar
Page 15 and 16:
Christine Paprotka, Mareike Rust, K
Page 17 and 18:
Markus Nees, Christian Hammann, Man
Page 19 and 20:
Rafal Archacki, T. Sarnowski, J. Ha
Page 21 and 22:
Christian Rohde, Yingying Zhang, To
Page 23 and 24:
Katerina Krizova, Miloslava Fojtova
Page 25 and 26:
Peter Hemmerich, Stefanie Weidtkamp
Page 27 and 28:
Ruxandra Farcas, Eberhard Schneider
Page 29 and 30:
Jürgen Geisel, Heike Schorr, Gunar
Page 31 and 32:
OLUSOLA DOKUN, WOLFGANG SCHULZ DNA
Page 33 and 34:
Christina Klaus, Daniela Kremer, Vi
Page 35 and 36:
Perrine Gaub, Andrea Tedeschi, Anto
Page 37 and 38:
Georgios J. Vlachojannis, Andreas M
Page 39 and 40:
Michael Michalkiewicz, Teresa Micha
Page 41 and 42:
Nadia Sellami, Sabine Adam-Klages,
Page 43 and 44:
Robert Liefke, Daniela Salat, Jörg
Page 45 and 46: Cordula Tschuch, Angela Schulz, Arm
Page 47 and 48: Soyoung Lim, Johannes Schulte, Hans
Page 49 and 50: Sandra Weiss, Ralf Gilsbach, Freder
Page 51 and 52: Irene Tiemann-Boege, Christina Curt
Page 53 and 54: Michael Haberland, Rusty Montgomery
Page 55 and 56: Eva Bartova, Abdrea Harnicarova, Ja
Page 57 and 58: Andrea Tedeschi, Tuan Nguyen, Radhi
Page 59 and 60: Philipp Rathert, Arunkumar Dhayalan
Page 61 and 62: Bastian Stielow, Alexandra Sapetsch
Page 63 and 64: Isabelle GUILLERET, Maria-Chiara OS
Page 65 and 66: Careen Katryniok, Bernd L. Sorg, Di
Page 67 and 68: Nathalie Jurisch, Bjoern Textor, Pe
Page 69 and 70: Fabio Mohn, Michael Weber, Michael
Page 71 and 72: Christian Schmidl, Maja Klug, Tina
Page 73 and 74: Stephanie Jungmichel, Christoph Spy
Page 75 and 76: Anette Tippelt Methyl Primer Expres
Page 77 and 78: Aditi Kanhere, Vingron Martin, Haas
Page 79 and 80: Renata Jurkowska, Da Jia, Sergey Ra
Page 81 and 82: Sascha Tierling, Yingying Zhang, Ch
Page 83 and 84: Franck COURT, Marion BANIOL, Hélè
Page 85 and 86: Andrea Felten, Peter Leister, Karl
Page 87 and 88: Niels Boeckel, Masamichi Koyanagi,
Page 89 and 90: Daniel Buszewicz, Marta Teperek, Pa
Page 91 and 92: Michael Grzendowski, Markus J. Riem
Page 93 and 94: Levin Böhlig, Kurt Engeland Regula
Page 95: Lin XU, Rozenn MENARD, Alexandre BE
Page 99 and 100: Amos Tanay Selection and mutation i
Page 101 and 102: Henriette Franz, Steven A. Jacobs,
Page 103 and 104: Günter Kahl, Carlos Molina Medina,
Page 105 and 106: Stephan Hupfer, Julia Brill, Cord-M
Page 107 and 108: Filip Senigl, Jiri Plachy, Jiri Hej
Page 109 and 110: Andrea Just, Falk Butter, Esther Li
Page 111 and 112: Marcus Buschbeck, Iris Uribesalgo,
Page 113 and 114: Andreas Thomae, Dagmar Pich, Jan Br
Page 115 and 116: Yamuna Gangadharan, Gary Karpen, Pa
Page 117 and 118: Christine Vogler, Tanja Waldmann, L
Page 119 and 120: Madeleine Meusburger, Mark Helm, Fr
Page 121 and 122: Akuma Divine Saningong, Peter Bayer
show all

Abstracts (poster) - Wissenschaft Online

You also want an ePaper? Increase the reach of your titles

Delete template?

Save as template?