Profilaksa DVT kod velikih ortopedskih operacija - Depol ...

20 - 22 September 2012, Opatija, Croatia
HUMAN TENDON PERIVASCULAR CELLS ARE MULTIPOTENT
AND EXPRESS EMBRYONIC STEM CELL ASSOCIATED MARKERS
Herbert Tempfer, Paracelsus Medical University, University Hospital Salzburg, Salzburg, Austria
Renate Gehwolf, Paracelsus Medical University, University Hospital Salzburg, Salzburg, Austria
Christine Lehner, Paracelsus Medical University, University Hospital Salzburg, Salzburg, Austria
Andrea Wagner, Paracelsus Medical University, University of Salzburg, Salzburg, Austria
Hans-Christian Bauer, Paracelsus Medical University, University Hospital Salzburg, Salzburg, Austria
Tendon-derived stem cells are commonly considered to be of mesenchymal origin, having the capacity to differentiate
into adipocytes, chondrocytes, osteoblasts and tendon cells. Earlier on we have shown that human tendon
perivascular cells express markers associated with neural stem cells such as Nestin and Musashi1.
Here we describe a so far unrecognized type of human tendon perivascular stem cells (hTPSC) expressing markers
commonly associated with embryonic stem cells (ESC). By immunohistochemistry and single cell PCR on human
tendon tissue we demonstrate that hTPSCs express Oct4, Nanog, Klf4, Cmyc and Sox2 in vivo. In cell culture, these
cells give rise to clonal spheroid cell aggregates harbouring cells expressing the stem cell markers mentioned and
markers associated with all three embryonic germ layers, such as Insulin and Glucagon, Collagens type 1 and 3 and
GFAP and Galactosyl ceramidase. In differentiation experiments, hTPSC can give rise to adipocytes, osteoblasts,
oligodendrocytes, astrocytes and insulin producing cells.
Despite their ESC-like marker expression, these cells do not form tumors upon injection into immunodeficient
mice.
These findings suggest that TPSC represent a more undifferentiated cell type than mesenchymal stem cells.
hTPSC may be a valuable source for future applications in tissue engineering and cell therapy.
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