Profilaksa DVT kod velikih ortopedskih operacija - Depol ...

92
2 st INTERNATIONAL CONFERENCE ON REGENERATIVE ORTHOPAEDICS
CELLULAR AND MOLECULAR ARCHITECTURE OF TENDON AND
LIGAMENT
Andrea Wagner, Paracelsus Medical Universtiy Salzburg, Tendon and Bone Regeneration, Salzburg,
Austria
Renate Gehwolf, Paracelsus Medical Universtiy Salzburg, Tendon and Bone Regeneration, Salzburg,
Austria
Corinna Hirzinger, Universtiy Hospital Salzburg, Traumatology and Sports Injuries, Salzbug, Austria
Milan Toljan, Sportmed Institute, Linz, Austria
Herbert Resch , Universtiy Hospital Salzburg, Traumatology and Sports Injuries, Salzbug, Austria
Hans-Cjristian Bauer, Paracelsus Medical Universtiy Salzburg, Salzburg, Austria
Tendons and ligaments are both dense connective tissues which resemble each other in many aspects but exert
different functions. While the cellular constituents of tendon tissue including tendon progenitor cells have been
well characterized, less information for ligament cells or progenitor cells in ligaments is available. We explore similarities
and differences between tendon and ligament cells at a molecular and biochemical level. Therefore we
used biopsy tissue of semitendinosus tendon (ST) and anterior cruciate ligament (ACL) since ruptured ACL often
is reconstructed by autologous ST grafts.
The expression of progenitor cell markers (Nestin, CD133, Scleraxis) in ACL and ST was analysed by immunhistochemistry
and quantitative RT-PCR. Our analyses revealed differences in the expression levels and expression patterns
of these marker molecules. We also determined the expression of extracellular matrix components (Collagen
type 1, Collagen type 3 and Aggrecan), matrix remodelling proteins (MMP-2, MMP-9, Lysyl oxidase) and Substance
P, a neurotransmitter in pain perception. The mRNA expression of Collagen type 1 and type 3, MMP-2 and Lysyl
oxidase was significantly higher in ACL than in ST. Substance P was only detectable in ACL, Scleraxis and Nestin
showed no differences. The ratio of Collagen type 1 to type 3 mRNA was significantly higher in ACL.
Our results may contribute to the understanding of tendon and ligament nature and origin, and will provide a clue
towards new therapeutic ways in ligament regeneration or reconstruction.
Supported: PMU-FFF E-09/09/051-BAH & ABT GmbH