Profilaksa DVT kod velikih ortopedskih operacija - Depol ...

20 - 22 September 2012, Opatija, Croatia
THE ROLE OF Nb IN OXIDIZED SURFACE OF A β-TiNb ALLOY ON
ITS OSTEOINTEGRATION
Marta Vandrovcova, Institute of Physiology AS CR, v.v.i, Prague, Czech Republic
Ivan Jirka, J. Heyrovský Institute of Physical Chemistry AS CR, v.v.i., Prague, Czech Republic
Otakar Frank, J. Heyrovský Institute of Physical Chemistry AS CR, v.v.i., Prague, Czech Republic
Zdenek Tolde, Czech Technical University in Prague, Faculty of Mechanical Engineering, Prague, Czech
Republic
Thomas Luxbacher, Anton Paar GmbH, Graz, Austria
Lucie Bacakova, Institute of Physiology AS CR, v.v.i , Prague, Czech Republic
Vladimir Stary, Czech Technical University in Prague, Faculty of Mechanical Engineerin, Prague, Czech
Republic
The most commonly used Ti-based materials for bone implant fabrication are the alloys composed from titanium,
aluminium and vanadium, particularly Ti6Al4V. These alloys fulfil many requirements for the implant material, i.e.
they are characterized by their beneficial mechanical and chemical properties and high biocompatibility. However,
substantial drawback of the TiAlV materials is the presence of small amounts of highly toxic Al and V oxide
species in their surface region, or release of cytotoxic Al and V ions. Great attention thus has been paid to develop
new materials which do not contain any toxic component. In this context, niobium is prospective as a non-toxic β
stabilizing agent.
In our study, the adhesion, growth and viability of human osteoblast-like MG63 cells on thermally oxidized surface
of titanium (Ti600) and β-titanium-niobium (TiNb600) alloy were investigated. We also compared the influence
-OH-group present on its surface (low concentration of OH-groups was observed after Piranha solution treatment).
The role of sterilization in boiling water on the surface chemistry of the samples and the presence of Nb
on the alloy surface on its interaction with cells was addressed. Sterilization of the samples caused extensive dehydroxylation
of their surfaces. However, the overall acidity of the Ti600 and TiNb600 samples before and after
sterilization is not affected by this treatment.
The differences observed between Ti600 and TiNb600 samples which may participate in higher “osteointegration”
can be summarized as follows: The surface of TiNb600 sample is more acidic. Moreover, the surface of TiNb600
sample is negatively charged at physiological pH (6–7). This charge is, however, smaller than the charge on the
surface of Ti600.
The authors found that TiNb600 alloys showed improved colonization with MG63 cells. This was documented by
significantly higher cell numbers on the TiNb600 (regardless of treatment by Piranha solution) compared with
pure Ti600. In addition cell viability on day 7 after seeding was significantly higher on TiNb600 than on Ti600. Thus,
niobium improves the mechanical properties of stainless steel, and it is suggested that addition of niobium to
metallic alloys (especially to titanium alloys) could be useful for the fabrication of biomedical materials required
definite mechanical features.
Supported by the Grant Agency of the Czech Republic (grant No. P108/10/1858)
Poster
89