Profilaksa DVT kod velikih ortopedskih operacija - Depol ...

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84 2 st INTERNATIONAL CONFERENCE ON REGENERATIVE ORTHOPAEDICS THE TENDON STEM/ PROGENITOR CELL NICHE: IS THERE A BLOOD TENDON BARRIER? Christine Lehner, University Hospital Salzburg, Salzburg, Austria Renate Gehwolf, Paracelsus Medical Univerity, Salzburg, Austria Andrea Wagner, Paracelsus Medical Univerity, Salzburg, Austria, University of Salzburg, Austria Hans-Christian Bauer, Paracelsus Medical Univerity, Salzburg, Austria Herbert Tempfer, Paracelsus Medical Univerity, Salzburg, Austria A major challenge for attempts to accelerate tendon healing is to understand the niche that regulates the differentiation of stem/ progenitor cells towards a tendon cell like phenotype. Along our attempts to characterize tendon cells we observed a population of perivascular cells expressing tendon- and neuronal stem cell associated markers. Moreover we observed that tendon endothelial cells express the tight junction (TJ) associated marker Occludin. We therefore hypothesise that tendon vessels form a barrier between blood and tendon tissue, establishing a privileged microenvironment. By immunohistochemistry, Lasermicrodissection, qRT-PCR and transmission electron microscopy we examined human biceps and semitendinosus tendons and mouse Achilles tendons for TJ associated markers and structures. Occludin, Claudin5 and ZO1 protein localize at the cell boundaries of tendon endothelial cells, as confirmed by confocal microscopy of isolated tendon vessels. Besides, these cells express mRNA encoding for the blood-brain barrier/ blood nerve barrier associated markers Occludin, Claudin1, Claudin5 and Claudin11. The finding of TJ- like structures in human tendons is confirmed by TEM images published by others. The finding of TJ- like structures in tendons may shed new light on the composition of the tendon niche. The functional properties and the role of this barrier in tendon de- and regeneration will have to be addressed by further studies. Poster
20 - 22 September 2012, Opatija, Croatia OSTEOGENIC POTENTIAL AND STEMNESS OF MESENCHYMAL STEM CELLS AFTER DEXAMETHASONE TREATMENT Igor Matić, Faculty of Science, University of Zagreb, Zagreb, Croatia Alan Ivković, Clinical Hospital Sveti Duh, Zagreb, Zagreb, Croatia Šime Brkić, Faculty of Science, University of Zagreb, Zagreb, Croatia Pavle Josipović, Faculty of Science, Univeristy of Zagreb, Zagreb, Croatia Ivan Karlak, Clinic for Traumatology, Zagreb, Zagreb, Croatia Marko Pećina, School of Medicine, University of Zagreb, Zagreb, Croatia Inga Marijanović, Faculty of Science, University of Zagreb, Zagreb, Croatia The purpose of the research was to improve osteogenic differentiation of human bone marrow derived-mesenchymal stem cells (hMSCs) and to evaluate the level of remaining undifferentiated stem cells in culture. Standard cell culture protocol includes continuous exposure to the dexamethasone which is not easy to reproduce in human body. Therefore, our experiment was designed to investigate the possibility of using short-term dexamethasone exposure in order to induce osteogenic differentiation. Human bone marrow-derived MSCs were expanded with FGF-2 supplemented media. Afterwards cells were cultured in osteogenic media (DMEM low glucose, 10% FBS, 10mM β-glycerophosphate, 5µg/ml ascorbic acid, 1mM piruvat) and treated with different concentrations of dexamethasone, both short-term and continuous. The differentiation status of the cells was estimated using several osteogenic markers - alkaline phosphatase (AP) activity, the bone sialoprotein (BSP) and dentin matrix protein 1 (DMP-1) mRNAs. The stemness of the cells was estimated by the presence of pluripotency markers - SRY (sex determining region Y)-box 2 (SOX2) and octamer-binding transcription factor 4 (OCT4). The measurements were conducted on the days 7 and 14 after osteogenic conditions had been introduced. Our results show that continuous treatment induces the highest level of osteogenesis, but 2 hour exposure also induced differentiation in comparison with control. Overall, 10-5 M concentration of dexamethasone was the most powerful in the short-term induction of osteogenesis. SOX2 has not been detected in hMSCs. OCT4 was expressed in undifferentiated pluripotent hMSCs and its expression subsequently went down, indicating that the differentiation process is proceeding Poster 85
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FINAL PROGRAM BOOK OF ABSTRACTS ORG
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CONTENTS 20 - 22 September 2012, Op
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Organized by: Croatian Academy of S
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CONFERENCE PROGRAMME: September 21
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17:15 - 17:25 Discussion 20:00 Soci
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20 - 22 September 2012, Opatija, Cr
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BOOK OF ABSTRACTS 20 - 22 September
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STEM CELLS FOR THE CURIOUS Stanimir
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