Profilaksa DVT kod velikih ortopedskih operacija - Depol ...

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2 st INTERNATIONAL CONFERENCE ON REGENERATIVE ORTHOPAEDICS
PLGA NANOFIBROUS MEMBRANES LOADED WITH
HYDROXYAPATITE OR DIAMOND NANOPARTICLES AS
SCAFFOLDS FOR BONE TISSUE ENGINEERING
Katarina Novotna, Institute of Physiology, Academy of Science of the Czech Republic, Prague, Czech
Republic
Martin Parizek, Institute of Physiology, Academy of Science of the Czech Republic, Prague, Czech Republic
Timothy Douglas, Polymer Chemistry and Biomaterials Group, Ghent University, Ghent, Belgium
Vera Lisa, Institute of Physiology, Academy of Science of the Czech Republic, Prague, Czech Republic
Lucie Bacakova, Institute of Physiology, Academy of Science of the Czech Republic, Prague, Czech
Republic
Various types of nanofiber scaffolds have been used in a wide range of tissue engineering applications, mainly
for their ability to mimic the architecture of natural extracellular matrix. Composites of nanofibrous polymers and
hydroxyapatite have been successfully tested for possible enhancement of bone regeneration.
We have evaluated the proliferation of osteoblast-like MG-63 cells on 2 types of poly(lactide-co-glycolide) (PLGA)
nanofibrous membranes, prepared by electrospinning using NanospiderTM device (Elmarco Ltd.). One type of
membranes was loaded with hydroxyapatite nanoparticles (PLGA-HAp), the second with diamond nanoparticles
(PLGA-ND), both about 23 wt% in dry PLGA. Besides the cell growth, we have also tested the possible immune activation
of the cells, manifested by secretion of tumor necrosis factor-alpha (TNF-alpha), measured in the cell culture
medium, and by concentration of intercellular adhesion molecule-1 (ICAM-1), measured in cell homogenates. As a
positive control for TNF-alpha production, the cells stimulated with lipopolysaccharide from Escherichia coli were
used. As MG-63 cells showed only a weak response to the lipopolysaccharide treatment, RAW 264.7 macrophages
were used for the evaluation as well.
The results have showed that both types of PLGA composites, as well as pristine PLGA represent a good support
for attachment and subsequent proliferation of the MG-63 cells. However a notable difference between these
PLGA composites was found in the TNF-alpha production. The concentration of TNF-alpha in the cell culture medium
taken from RAW 264.7 cells grown on PLGA-HAp was significantly higher than on PLGA-ND and pristine PLGA,
which makes PLGA-ND constructs suitable for its use in bone tissue engineering and for further research.
Supported by the Academy of Sciences of the CR (grant No. IAAX00100902) and the Czech Science Foundation
(grants No. P108/11/0794 and P108/12/G108).
Poster