International Journal of Noni Research - Noni Family

41 Intl. J. Noni Res. 2007, 2(1-2)
Result and discussion
Shoot initiation
Both Ms and WP media were used along with various combinations of
Cytokinins and Auxin. Axillary bud sprouting were observed in both the
basal media with 2.0 mg/l BAP alone. (Fig 3 & 4)
Multiplication in-vitro
After four weeks of axillary bud sprouting, initiated young shoots with the
nodal region were transferred to multiplication media. BAP 4.0 mg/l, along
with IAA 0.5 mg/l have produced 3-5 multiple shoots within 4-6 weeks after
subculture (Fig 5). Both MS basal and WP were found equally good enough
for culture multiplication. Culture were allowed to grow at 26+2 o
C
temperature and humidity at 55 + 10%, photoperiod 16 hrs light and 8 hrs
dark and light intensity of 2000 lux.
In-vitro rooting
J. Subramani et al. Micropropagation of Morinda citrifolia L.
Fig.3
Fig.5
Fig.4
Out of MS and WP basal media used, MS basal with 1.0 mg /l IBA and
0.5 mg /l NAA have produced enough roots in vitro (Fig.6). 4 to 6 cm
long shoots were removed separately from the multi culture and
inoculated in the rooting media. MS media with 1.0 mg /l IBA and 0.5 mg
/1 NAA were used in the rooting media. Individual plants with roots were
transferred to green house for hardening.