International Journal of Noni Research - Noni Family

J. Subramani et al. Micropropagation of Morinda citrifolia L.
Hardening at green house
Well rooted plants were transferred to pro trays (98 cavities) containing
coco-peat (Fig. 7). After hardening for 5-6 weeks, young plantlets were
transferred to nursery stage (Fig.8). Green house temperature was maintained
at 32 + 4 0 C and humidity 60 + 20%.
Callus Initiation
Amount of 2 mg/1 2, 4 –D in both MS and WP media were found to be ideal
for callus initiation (Fig. 9). Calli were further maintained in media containing
2 mg /1 2,4-D (Fig.10).
Callus regeneration
Fig.6
Fig.7 Fig.8
Fig.9 Fig.10
a. Callus from MS/WP media were further sub-cultured on media (MS/WP)
containing 4 mg BAP, 0.5 mg/l kinetin that resulted in formation of single
shoot in 25 days (Fig. 11).
Intl. J. Noni Res. 2007, 2(1-2) 42