International Journal of Noni Research - Noni Family
International Journal of Noni Research - Noni Family
International Journal of Noni Research - Noni Family
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D.R.Singh<br />
Jai Sunder<br />
R.C.Srivastava<br />
Authors’ affiliation :<br />
D.R.Singh<br />
Jai Sunder<br />
R.C.Srivastava<br />
Central Agricultural <strong>Research</strong> Institute<br />
Indian council <strong>of</strong> Agricultural <strong>Research</strong><br />
Port Blair, Andaman and Nicobar Islands<br />
India - 744 101.<br />
drsingh1966@yahoo.com<br />
Correspondence to :<br />
D.R.Singh<br />
Central Agricultural <strong>Research</strong> Institute<br />
Indian council <strong>of</strong> Agricultural <strong>Research</strong><br />
Port Blair, Andaman and Nicobar Islands<br />
India - 744 101.<br />
drsingh1966@yahoo.com<br />
75 Intl. J. <strong>Noni</strong> Res. 2007, 2(1-2)<br />
Peptide and Mineral pr<strong>of</strong>ile <strong>of</strong><br />
Morinda citrifolia fruits and leaves<br />
Keywords : Peptide pr<strong>of</strong>ile, leaves, fruits M.citrifolia<br />
Abstract : Morinda citrifolia (noni) has a long history related to medical<br />
uses in Southeast Asian countries. Today, noni grows in the majority <strong>of</strong> the<br />
southern Pacific areas, in India, the Caribbean, South America and the<br />
West Indies. In order to obtain better understanding <strong>of</strong> the medicinal<br />
characteristics <strong>of</strong> the noni fruits and leaves, the peptide pr<strong>of</strong>ile and the<br />
mineral compositions <strong>of</strong> the raw juice extracted from M. citrifolia fruits<br />
and leaves were determined. Morinda citrifolia fruit and leaves were<br />
subjected to dry ashing for the preparation <strong>of</strong> acid-mineral extract for<br />
micronutrients and macronutrients analysis by atomic absorption<br />
spectrophotometer. For analysis <strong>of</strong> peptide pr<strong>of</strong>ile the water extraction <strong>of</strong><br />
the M.citrifloia fruits and leaves were done and the extract was used for<br />
HPLC analysis. The peptide pr<strong>of</strong>ile <strong>of</strong> the Morinda citrifoilia fruits and<br />
leaves were studied by using high performance liquid chromatography<br />
(HPLC). The concentration <strong>of</strong> micro and macro mineral <strong>of</strong> M.citrifolia<br />
leaves was found to be more than fruits. The HPLC analysis <strong>of</strong> the peptide<br />
pr<strong>of</strong>ile <strong>of</strong> the M.citrifolia revealed the following peptide in various<br />
concentration such as Gly-Tyr, val-Tyr-Val, Meth-Enkaphalin and Leu-<br />
Enkaphalin. Overall, the peptide concentration <strong>of</strong> the leaf extract was<br />
found to be more than the fruit extract. High level <strong>of</strong> peptide Val-Tyr-Val<br />
was found in leaf extract (15.856 ppm) while in fruit the peptide Leuenkephalin<br />
was maximum (3.697 ppm). The peptide analysis <strong>of</strong> the<br />
M.citrifolia may be useful in studying various alkaloids present in the<br />
fruits and leaves and will be <strong>of</strong> use in studying the effect <strong>of</strong> Morinda<br />
extract in immune system regulation.<br />
Introduction<br />
Andaman & Nicobar Islands have a vast variety and diversity in tropical<br />
underutilized fruits. Their plant parts have a close association with local beliefs<br />
and rituals and are used in health care needs in rich ethnic life <strong>of</strong> people. The<br />
interesting fact is that the tribes i.e., Nicobari tribes <strong>of</strong> these islands have very<br />
long association with noni plant, as they have been using different parts as<br />
medicine for different purposes. They consume its fruits raw with salt as well<br />
as cooked vegetables (Singh et al., 2005).