NSLS Activity Report 2006 - Brookhaven National Laboratory

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PLANT STUDIES REVEAL HOW, WHERE SEEDS STORE IRON Dartmouth College issued the following press release detailing the storage of iron in plant seeds. These findings, which could lead to ways to prevent iron deficiency and malnutrition, were discovered at the National Synchrotron Light Source. At beamline X26A, researchers used fluorescence tomography, an x-ray imaging technique that allows scientists to “see” the distribution of elements inside the seeds without destroying them. Dartmouth biologists are leading a research team Dartmouth researchers Sun A. Kim, Mary Lou Guerinot, and Tracy Punshon (photo credit, Joseph Mehling, Dartmouth College). that has learned where and how some plant seeds store iron, a valuable discovery for scientists working to improve the iron content of plants. This research helps address the worldwide issue of iron deficiency and malnutrition. Their findings were published in the November 24, 2006 edition of the journal Science. The team found that iron is stored in the developing vascular system of the seed of Arabidopsis, a model plant used in research; in particular, iron is stored in the vacuole, a plant cell’s central storage site. The researchers also learned that this localization is dependent on a protein called VIT1, shown to transport iron into the vacuole. Dartmouth Professor of Biological Sciences Mary Lou Guerinot, the principal investigator on the study, says, “Iron deficiency is the most common human nutritional disorder in the world today, afflicting more than three billion people worldwide. Most of these people rely on plants for their dietary iron. However, plants are not high in iron, and the limited availability of iron in the soil can limit plant growth. Our study certainly suggests that iron storage in the vacuole is a promising and, before now, 2-10 largely unexplored target for increasing the iron content of seeds. Such nutrient-rich seeds would benefit both human health and agricultural productivity.” The researchers combined traditional mutant analysis (turning on and off the VIT1 protein) with a powerful x-ray imaging technique to create a map of where iron is localized in the seed. Guerinot was surprised by the finding because most studies on iron storage focus on the protein ferritin. This paper reveals how important it is to look beyond ferritin to understand how iron is stored by plants. The researchers say their study suggests that the stored iron in the vacuole is an important source of iron for developing seedlings. Seedlings that do not express the VIT1 protein grow poorly when iron is limited. “We have demonstrated the usefulness of synchrotron x-ray fluorescence microtomography to look inside a seed,” says Guerinot. “This technique is noninvasive and requires no sample preparation. We think our work will open the way for many more biologists to use this technique to examine the spatial distribution of metals in samples of interest.” The imaging was carried out at the Department of Energy’s National Synchrotron Light Source at Brookhaven National Laboratory. Other authors on the paper include Sun A. Kim and Tracy Punshon, both postdoctoral fellows at Dartmouth, Antonio Lanzirotti of the University of Rendered three-dimensional tomographic reconstruction of the iron (Fe) fl uorescence from a wild type Arabidopsis thaliana seed (approx. 100 µm wide seed). In this seed, the tomographic reconstruction demonstrates that Fe is localized around the embryonic provasculature of the radicle and cotyledons. Using fl uorescence microtomography, the research team demonstrated the vital role the gene vacuolar iron transporter 1 serves in controlling Fe homeostasis in plants.
Chicago, Liangtao Li and Jerry Kaplan from the University of Utah School of Medicine, José Alonso with North Carolina State University, and Joseph Ecker with the Salk Institute for Biological Studies. This research was supported by grants from the National Science Foundation and the National Institutes of Health. Tracy Punshon was supported by a training fellowship provided by the National Institute of Environmental Health Sciences Superfund Basic Research Program Project through Dartmouth’s Center for Environmental Health Sciences. For more information, see: S.A. Kim, T. Punshon, A. Lanzirotti, L. Li, J.M. Alonso, J.R. Ecker, J. Kaplan, and M.L. Guerinot, "Localization of Iron in Arabidopsis Seed Requires the Vacuolar Membrane Transporter VIT1," Science, 314, 1295-1298 (2006). — Sue Knapp, Dartmouth University 2-11 FEATURE HIGHLIGHTS
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Energy Secretary Samuel Bodman (fro
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SAFETY REPORT Andrew Ackerman ESH&Q
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FACILITY FACTS & FIGURES The Nation
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