HiTec Zang GmbH -- Main Catalogue 2009/10

38
The demand for equal cultivation conditions for
micro-organisms in normal shaking flasks and in
RAMOS-Measuring flasks was given priority in the
development of the plant. For this reason, the design
of the flask was devised, in regard to the gas and
hydrodynamics, so that it accords to a normal
shaking flask in the bottom part containing liquid.
In addition, a method was developed which enables
to determine the oxygen transfer merely out of the
variation of the oxygen partial pressure in the head
space of the measuring flasks (no invasive
electrodes). The system is patented.
To ensure equivalent cultivation conditions, the gas
composition above the culture liquid in the flasks of
the measuring plant is adjusted identically to the
composition in a normal flask. For this purpose, the
gas flow is set client-specifically (depending on the
type of sterile seals used).
The system handling can be learned very easily and
quickly. Within a short period of time, you will not be
able to imagine to work without RAMOS
The RAMOS System
Fermentation Technology
The RAMOS system fits on a normal benchtop. It is
delivered completely installed with flasks, sensors,
shaker, incubator, and control PC including an
uninterrupted power supply system.
For the familiarisation with the new methods, we
offer one to two-day seminars and training
programmes.
Measurement of the Oxygen Transfer Rate
The oxygen transfer rate is the most suitable and
most universal variable to characterise and quantify
the physiological condition and the activity of an
aerobe culture because almost every metabolic
activity is combined with oxygen consumption.
The online measurement of the oxygen transfer rate
in shaken bioreactors is state-of-the-art, however it
is technically very laborious. The determination of
the oxygen transfer rate in shaken bioreactors
(shaking flasks) under sterile conditions has yet not
been possible. RAMOS closes this gap and
therefore combines the advantages of shaking flasks
(little space required, cost-efficient, the possibility of
parallel operation) with those of a stirred bioreactor
(online-measurement).
OTR- Phenomena
The figure gives a schematic selection of biological
phenomena and their effect upon the oxygen
transfer rate (OTR). The diagrams show how the
behaviour of a microbial culture can be
systematically analysed with the help of the
measuring curves delivered by RAMOS.
Measuring of the Carbon Dioxide Transfer Rate
Next to the oxygen transfer rate, the carbon dioxide
transfer rate (CTR) and the respiratory quotient (RQ)
are important sources of information for
microbiologists.
The temporal development of OTR, CTR and RQ
can be clearly monitored online.
In addition, further process parameters are
calculated hereof. The evaluation can be extended
according to user specifications.
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