HiTec Zang GmbH -- Main Catalogue 2009/10

40
Fermentation Technology
Feeding and Sample Drawing in Shaking Flasks
Fluid-Train ® -Technology
Pharmaceutically relevant products, such as insulin,
antibodies, and many more, are synthesised by
microorganisms and cell cultures in bioreactors.
With the Fed-batch regulation, the pro- or eukaryotic
cells are fed on a defined and regulated basis. Up to
now, the use of this procedure was restricted to
large-scale production to achieve optimal biomass,
respectively product yield.
RAMOS-Fed-batch provides fully automatic Fedbatch
fermentation in shaking flasks. The parallel
technology and the approved measurement
procedure of the RAMOS were expanded by an
automated dosing system.
RAMOS-Fed-batch makes controlled feeding
possible and regulates the dosing rates based on
the oxygen transfer rate (OTR) or respiration
quotient (RQ). The cell number-independant key
parameter RQ gives information on the physiological
condition and is evaluated from the oxygen and
carbon dioxide transfer rate (CTR) and thus allows a
metabolism-dependant regulated dosing. The
targeted regulation of the ideal RQ value can
therefore be utilised to achieve maximum product
yield. Based on these results, a feeding algorithm
was developed, which enables to to keep a
physiological condition constant without drawing any
samples and analytics. The dosing amount is
precisely recorded by the software.
Technical Data
The innovative Fluid-Train Dosing and Sample
Drawing Technology (FTT) ensures a comminglingfree
dosing and sample drawing conducted through
merely one tube. Consequently, it is possible to
seperatly and sterilely dose two or optionally more
different media into each individual shaking flask.
The combination with the RAMOS measuring data
enables the feeding of the cultures with exactly the
required amount of substrate, which leads to ideal
culture growth and ideal production, respectively.
The diagram show a dosing control upon the OTR
value. The starting set point of 0,004 mol/l*h is being
reduced after 45 hrs. to 0,002 mol/l*h. After approx.
ca. 60, 90 and 100 std. the feeding of media will be
temporarily interrupted.
Parallel, up to 24 samples are, either event or
schedule-controlled, are drawn from the culture
containers and deposited (AutoSam-s). This makes
unattended operation over night or weekend
possible.
Rinsing and cleaning cycles warrant a
contamination-free operation. The Fluid-Train
Technology is applied for the fermentation of proand
eukaryotic organisms.
Receiver Bottle 2 Feed-Media Bottles, 1 Bottle NaOH, 1 Bottle ultra-pure Water
Dosing Rate 1-2 ml per flask and dosing cycle, cycle period
Order Code Description
VA-RAMOSFTT8 Program-controlled dosing and sample drawing , 8 channels including 4 receivers
VA-RAMOSCON8 RAMOS Dosing regulation for control variable = OTR, CTR or RQ, 8 channels
RAMOSFTT8 requires an AutoSam sampling device IL-AUTOSAM-xx and if necessary a cooling
incubatro IL-AUTOSAM-KL.
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