Proteomica - CusMiBio - UniversitÃ degli Studi di Milano

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Trypsin yields 47 peptides (theoretically)! Peptide masses in Da:! 501.3! 533.3! 544.3! 545.3! 614.4! 634.3! ! ! 674.3! 675.4! 701.4! 726.4! 822.4! 855.5! ! ! 861.4! 879.4! 921.5! 953.4! 974.5! 988.5! ! ! 1000.6! 1196.6! 1217.6! 1228.5! 1232.6! 1233.7! ! ! 1249.6! 1249.6! 1344.7! 1455.8! 1484.6! 1514.8! ! ! 1582.9! 1583.9! 1616.8! 1726.7! 1759.9! 1775.9! ! ! 1790.6! 1853.9! 1869.9! 2286.2! 2302.2! 2317.2! ! ! 2419.2! 2526.4! 2542.4! 3329.6! 4211.4! ! ! http://us.expasy.org/tools/peptide-mass.html!
1007! 1199! 2211 (trp)! 609! 450! 698! 1940 (trp)! 2098! 500 1000 1500 2000 2500!
Page 1 and 2: Cos’è il PROTEOMA! Si definisce
Page 3 and 4: B. LEWIN, IL GENE - Edizione compat
Page 5 and 6: Why proteomics • La velocità di
Page 7 and 8: PROTEOMICS APPLICATIONS Proteogenom
Page 9: pI= carica nulla!
Page 14 and 15: Metodo analitico per misurare esatt
Page 17: 337 nm UV laser! Fluid (no salt)! +
Page 21: 53! 6! 54! 3 ! 4! 5! 1! 2! 10! 8! 9
Page 24 and 25: Sequence! Mass (M+H)! Mass Spectrum
Page 26 and 27: Enzima molto stabile Lavora in un
Page 30 and 31: Query Masses Database Mass List Res
Page 32 and 33: 2DE gel Intact protein Experimental
Page 34 and 35: P.M. 43 KDa Componente del ribosom
Page 36 and 37: The secreted material was obtained
Page 39 and 40: MODIFICAZIONI DI PEPTIDI E PROTEINE
Page 41 and 42: Lysine specific histone demethylase
Page 45 and 46: E’ importante identificare il sit
Page 47: La proteina è un biomarcatore di p
Page 55: Fractionation &! Isolation! 2-DE! L
Page 58 and 59: 337 nm UV laser! cyano-hydroxy! cin
Page 60: BIBLIOGRAFIA! Methods in Molecular
Page 63 and 64: CID: Collision-Induced Dissociation
Page 65 and 66: CID: Collision-Induced Dissociation
Page 68: His 6 - Cdc34 S130A 123 SVLISIVA 13

Proteomica - CusMiBio - UniversitÃ degli Studi di Milano

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