Proteomica - CusMiBio - UniversitÃ degli Studi di Milano

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Query Masses Database Mass List Results! 450.2201! 609.3667! 698.3100! 1007.5391! 1199.4916! 2098.9909! 450.2017 (P21234) ! 609.2667 (P12345) ! 664.3300 (P89212) ! 1007.4251 (P12345)! 1114.4416 (P89212)! 1183.5266 (P12345)! 1300.5116 (P21234) ! 1407.6462 (P21234)! 1526.6211 (P89212)! 1593.7101 (P89212) ! 1740.7501 (P21234) ! 2098.8909 (P12345)! 2 Unknown masses! 1 hit on P21234! 3 hits on P12345! Conclude the query! protein is P12345!
PeptIdent (ExPasy)! Mascot (Matrix Science)! MS-Fit (Prospector; UCSF)! ProFound (Proteometrics)! MOWSE (HGMP)! Human Genome Mapping Project! Mascot! theoretical! Protein ID! experimental!
Page 1 and 2: Cos’è il PROTEOMA! Si definisce
Page 3 and 4: B. LEWIN, IL GENE - Edizione compat
Page 5 and 6: Why proteomics • La velocità di
Page 7 and 8: PROTEOMICS APPLICATIONS Proteogenom
Page 9: pI= carica nulla!
Page 14 and 15: Metodo analitico per misurare esatt
Page 17: 337 nm UV laser! Fluid (no salt)! +
Page 21: 53! 6! 54! 3 ! 4! 5! 1! 2! 10! 8! 9
Page 24 and 25: Sequence! Mass (M+H)! Mass Spectrum
Page 26 and 27: Enzima molto stabile Lavora in un
Page 28 and 29: Trypsin yields 47 peptides (theoret
Page 32 and 33: 2DE gel Intact protein Experimental
Page 34 and 35: P.M. 43 KDa Componente del ribosom
Page 36 and 37: The secreted material was obtained
Page 39 and 40: MODIFICAZIONI DI PEPTIDI E PROTEINE
Page 41 and 42: Lysine specific histone demethylase
Page 45 and 46: E’ importante identificare il sit
Page 47: La proteina è un biomarcatore di p
Page 55: Fractionation &! Isolation! 2-DE! L
Page 58 and 59: 337 nm UV laser! cyano-hydroxy! cin
Page 60: BIBLIOGRAFIA! Methods in Molecular
Page 63 and 64: CID: Collision-Induced Dissociation
Page 65 and 66: CID: Collision-Induced Dissociation
Page 68: His 6 - Cdc34 S130A 123 SVLISIVA 13

Proteomica - CusMiBio - UniversitÃ degli Studi di Milano

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