Proteomica - CusMiBio - UniversitÃ degli Studi di Milano

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Phosphorylation of Sic1 by CK2 in budding yeast cells (A) 2-D silver-stained gel of Sic1 immunopurified from elutriated cells with 0.90 of budding index (yeast strain 4245 Sic1-HA). Spots 1 and 2 are indicated (upper panel). After treatment with alkaline phosphatase only the spot 1 was detected (lower panel). (B) MALDI-TOF spectra of the spot 2 of Sic1 pooled from five individual gels. A peak at 2235.65 m/z corresponding to peptide 198–216 was indicated. Inset shows that after phosphatase treatment the non-phosphorylated peak at 2156.55 m/z was identified.   BBRC 346 (2006) 786-793!
Lysine specific histone demethylase 1 (LSD1): tissue specificity and phosphorylation pattern of the LSD1-8a isoform! LSD1 contributes to chromatin remodeling by specifically removing methyl groups from Lys4 of histone H3. The E8a containing isoforms are detected only in brain tissues and contributes to neurite morphogenesis! LSD1-2a/8a! LSD1-2a! LSD1-8a! LSD1! 8a 2a! 8a! Asp Thr Val Lys! 1-876aa! 1-872aa! 1-856aa! 1-852aa!
Page 1 and 2: Cos’è il PROTEOMA! Si definisce
Page 3 and 4: B. LEWIN, IL GENE - Edizione compat
Page 5 and 6: Why proteomics • La velocità di
Page 7 and 8: PROTEOMICS APPLICATIONS Proteogenom
Page 9: pI= carica nulla!
Page 14 and 15: Metodo analitico per misurare esatt
Page 17: 337 nm UV laser! Fluid (no salt)! +
Page 21: 53! 6! 54! 3 ! 4! 5! 1! 2! 10! 8! 9
Page 24 and 25: Sequence! Mass (M+H)! Mass Spectrum
Page 26 and 27: Enzima molto stabile Lavora in un
Page 28 and 29: Trypsin yields 47 peptides (theoret
Page 30 and 31: Query Masses Database Mass List Res
Page 32 and 33: 2DE gel Intact protein Experimental
Page 34 and 35: P.M. 43 KDa Componente del ribosom
Page 36 and 37: The secreted material was obtained
Page 39: MODIFICAZIONI DI PEPTIDI E PROTEINE
Page 45 and 46: E’ importante identificare il sit
Page 47: La proteina è un biomarcatore di p
Page 55: Fractionation &! Isolation! 2-DE! L
Page 58 and 59: 337 nm UV laser! cyano-hydroxy! cin
Page 60: BIBLIOGRAFIA! Methods in Molecular
Page 63 and 64: CID: Collision-Induced Dissociation
Page 65 and 66: CID: Collision-Induced Dissociation
Page 68: His 6 - Cdc34 S130A 123 SVLISIVA 13

Proteomica - CusMiBio - UniversitÃ degli Studi di Milano

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