Proteomica - CusMiBio - UniversitÃ degli Studi di Milano

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Protein Mixture! Tandem Mass Spectrometer! Digestion! 2D Chromatography! Peptide Mixture! RP! SCX! > 1,000 Proteins Identified! MS/MS Spectrum! SEQUEST ® ! DTASelect & Contrast!
Page 1 and 2:
Cos’è il PROTEOMA! Si definisce
Page 3 and 4:
B. LEWIN, IL GENE - Edizione compat
Page 5 and 6: Why proteomics • La velocità di
Page 7 and 8: PROTEOMICS APPLICATIONS Proteogenom
Page 9: pI= carica nulla!
Page 14 and 15: Metodo analitico per misurare esatt
Page 17: 337 nm UV laser! Fluid (no salt)! +
Page 21: 53! 6! 54! 3 ! 4! 5! 1! 2! 10! 8! 9
Page 24 and 25: Sequence! Mass (M+H)! Mass Spectrum
Page 26 and 27: Enzima molto stabile Lavora in un
Page 28 and 29: Trypsin yields 47 peptides (theoret
Page 30 and 31: Query Masses Database Mass List Res
Page 32 and 33: 2DE gel Intact protein Experimental
Page 34 and 35: P.M. 43 KDa Componente del ribosom
Page 36 and 37: The secreted material was obtained
Page 39 and 40: MODIFICAZIONI DI PEPTIDI E PROTEINE
Page 41 and 42: Lysine specific histone demethylase
Page 45 and 46: E’ importante identificare il sit
Page 47: La proteina è un biomarcatore di p
Page 55: Fractionation &! Isolation! 2-DE! L
Page 59 and 60: Normal! Tumor!
Page 62 and 63: CID: Collision-Induced Dissociation
Page 64 and 65: CID: Collision-Induced Dissociation
Page 66: CID: Collision-Induced Dissociation
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Proteomica - CusMiBio - UniversitÃ degli Studi di Milano

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