Proteomica - CusMiBio - UniversitÃ degli Studi di Milano

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Un solo approccio….. A Bruker MALDI TOF-TOF autoflex III * ThermoFisher LTQ Orbitrap Velos ETD and nano HPLC Ultimate 3000 Dionex Procise Applied Biosystems
pI= carica nulla!
Page 1 and 2: Cos’è il PROTEOMA! Si definisce
Page 3 and 4: B. LEWIN, IL GENE - Edizione compat
Page 5 and 6: Why proteomics • La velocità di
Page 7: PROTEOMICS APPLICATIONS Proteogenom
Page 14 and 15: Metodo analitico per misurare esatt
Page 17: 337 nm UV laser! Fluid (no salt)! +
Page 21: 53! 6! 54! 3 ! 4! 5! 1! 2! 10! 8! 9
Page 24 and 25: Sequence! Mass (M+H)! Mass Spectrum
Page 26 and 27: Enzima molto stabile Lavora in un
Page 28 and 29: Trypsin yields 47 peptides (theoret
Page 30 and 31: Query Masses Database Mass List Res
Page 32 and 33: 2DE gel Intact protein Experimental
Page 34 and 35: P.M. 43 KDa Componente del ribosom
Page 36 and 37: The secreted material was obtained
Page 39 and 40: MODIFICAZIONI DI PEPTIDI E PROTEINE
Page 41 and 42: Lysine specific histone demethylase
Page 45 and 46: E’ importante identificare il sit
Page 47: La proteina è un biomarcatore di p
Page 55: Fractionation &! Isolation! 2-DE! L
Page 58 and 59:
337 nm UV laser! cyano-hydroxy! cin
Page 60:
BIBLIOGRAFIA! Methods in Molecular
Page 63 and 64:
CID: Collision-Induced Dissociation
Page 65 and 66:
CID: Collision-Induced Dissociation
Page 68:
His 6 - Cdc34 S130A 123 SVLISIVA 13
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Proteomica - CusMiBio - UniversitÃ degli Studi di Milano

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