Proteomica - CusMiBio - Università degli Studi di Milano
Proteomica - CusMiBio - Università degli Studi di Milano
Proteomica - CusMiBio - Università degli Studi di Milano
- No tags were found...
You also want an ePaper? Increase the reach of your titles
YUMPU automatically turns print PDFs into web optimized ePapers that Google loves.
Phosphorylation of Sic1 by CK2 in bud<strong>di</strong>ng yeast cells<br />
(A) 2-D silver-stained gel of Sic1<br />
immunopurified from elutriated cells<br />
with 0.90 of bud<strong>di</strong>ng index (yeast strain<br />
4245 Sic1-HA). Spots 1 and 2 are<br />
in<strong>di</strong>cated (upper panel). After<br />
treatment with alkaline phosphatase<br />
only the spot 1 was detected (lower<br />
panel).<br />
(B) MALDI-TOF spectra of the spot 2 of<br />
Sic1 pooled from five in<strong>di</strong>vidual gels. A<br />
peak at 2235.65 m/z correspon<strong>di</strong>ng to<br />
peptide 198–216 was in<strong>di</strong>cated. Inset<br />
shows that after phosphatase<br />
treatment the non-phosphorylated peak<br />
at 2156.55 m/z was identified. <br />
BBRC 346 (2006) 786-793!
Change language