Proteomica - CusMiBio - UniversitÃ degli Studi di Milano

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The secreted material was obtained by electrostimulation of the parasites in a 50% PBS solution using 40 volts electric field and 2 Hz frequency! Protein Putative secreted salivary protein Prefol<strong>di</strong>n Putative esophageal gland cell secretory protein Esterase Gland-specific protein 10! 100! To solubilize the adhesive material 30 μL of lysis buffer (7M Urea, 2M Tiourea, 4% Chaps, 40mM TrisHCl) were added to each test tube! 3 pI! 10! 7! 1! 6! 5! 32! 8! 10!1 9! 1 33! 19! 20! 21! 28!
Quali sono le proprietà della mia proteina!
Page 1 and 2: Cos’è il PROTEOMA! Si definisce
Page 3 and 4: B. LEWIN, IL GENE - Edizione compat
Page 5 and 6: Why proteomics • La velocità di
Page 7 and 8: PROTEOMICS APPLICATIONS Proteogenom
Page 9: pI= carica nulla!
Page 14 and 15: Metodo analitico per misurare esatt
Page 17: 337 nm UV laser! Fluid (no salt)! +
Page 21: 53! 6! 54! 3 ! 4! 5! 1! 2! 10! 8! 9
Page 24 and 25: Sequence! Mass (M+H)! Mass Spectrum
Page 26 and 27: Enzima molto stabile Lavora in un
Page 28 and 29: Trypsin yields 47 peptides (theoret
Page 30 and 31: Query Masses Database Mass List Res
Page 32 and 33: 2DE gel Intact protein Experimental
Page 34 and 35: P.M. 43 KDa Componente del ribosom
Page 39 and 40: MODIFICAZIONI DI PEPTIDI E PROTEINE
Page 41 and 42: Lysine specific histone demethylase
Page 45 and 46: E’ importante identificare il sit
Page 47: La proteina è un biomarcatore di p
Page 55: Fractionation &! Isolation! 2-DE! L
Page 58 and 59: 337 nm UV laser! cyano-hydroxy! cin
Page 60: BIBLIOGRAFIA! Methods in Molecular
Page 63 and 64: CID: Collision-Induced Dissociation
Page 65 and 66: CID: Collision-Induced Dissociation
Page 68: His 6 - Cdc34 S130A 123 SVLISIVA 13

Proteomica - CusMiBio - UniversitÃ degli Studi di Milano

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