CHUNG, SOONKYU, Ph. D. Mechanisms by Which Conjugated ...

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Lagathu, C., Bastard, J., Auclair, M., Maachi, M., Capeau, J. and Caron, M. (2003) Chronic interleukin-6 (IL-6) treatment increased IL-6 secretion and induced insulin resistance in adipocyte: prevention by rosiglitazone. Biophys. Biochem. Res. Commun. 311:372-379. Landos, C., Brasaemle, D., Schultz, C, Segrest, J., and Kimmel, A. (1999) Perilipins, ADRP, and other proteins that associate with intracellular lipids droplets in animal cells. Cell and Dev. Biol. 10: 51-58. Lappas, M., Yee, K., Permezel, M., and Rice, G.E. (2005) Sulfasalazine and BAY 11- 7082 interfere with the nuclear factor-kappa B and I kappa B kinase pathway to regulate the release of proinflammatory cytokines from human adipose tissue and skeletal muscle in vitro. Endocrinol. 146, 1491-1497 Larsen, T.M., Toubro, S., and Astrup, A. (2003) Efficacy and safety of dietary supplements containing CLA for the treatment of obesity: evidence from animal and human studies. J. Lipid Res. 44: 2234-2241 Lau, D.C., Dhillon, B., Yan, H., Szmitko, P.E., and Verma, S. (2005) Adipokines: molecular links between obesity and atherosclerosis. Am. J. Physiol. Heart Circ. Physiol. 288, H2031-2041 Lee, J.Y., Ye, J., Gao, Z., Youn, H.S., Lee, W.H., Zhao, L., Sizemore, N., and Hwang D.H. (2003) Reciprocal modulation of toll-like receptor-4 signaling pathways involving MyD88 and phosphatidylinositol 3-kinase/AKT by saturated and polyunsaturated fatty acids. J. Biol. Chem. 278: 37041-37051 Lee, K., Kritchevsky, D. and Pariza, M. (1994) Conjugated linoleic acid and atherosclerosis in rabbits. Atherosclerosis 108:19-25 Li, Z.W. (1999) The IKK beta subunit of IKK is essential for nuclear factor kappa B activation and prevention of apoptosis. J. Exp. Med. 189: 1839-1845 Li, X., Massa, P.E., Hanidu, A., Peet, G.W., Aro. P., Savitt, A., Mische, S., Li, J., and Marcu, K.B. (2002) IKKalpha, IKKbeta, and NEMO/IKKgamma are each required for the NF-kappa B-mediated inflammatory response program. J. Biol. Chem. 277, 45129- 45140 Lin, Y., Lee, H., Berg, A.H., Lisanti, M.P., Shapiro, L., and Scherer, P.E. (2000) The lipopolysaccharide-activated toll-like receptor (TRL)-4 induces synthesis of the closely related receptor TRL-2 in adipocytes. J. Biol. Chem. 278: 37041-37051 143
Loscher, C.E., Draper, E., Leavy, O., Kelleher, D., Mills, K.H., Roche, H.M.(2005) Conjugated linoleic acid suppresses NF-kappa B activation and IL-12 production in dendritic cells through ERK-mediated IL-10 induction. J. Immunol. 175:4990-8. Luongo, D., Bergamo, P., and Rossi, M. (2003) Effects of conjugated linoleic acid on growth and cytokine expression in Jurkat T cells. Immun. Lett. 90: 195-202 Martinez-Botas, J.J., Anderson, D., Tessier, A., Lapillonne, B., Chang, M., Quast, D., Gorenstein, K., Chen, L., and Chan. (2000) Absence of perilipin results in leanness and reverses obesity in Lepr (db/db) mice. Nat. Genet. 26: 474-479. May, M., D’Acquisto, J., Madge, F., Glockner, L.A., Pober, J., Sanker, J.S. and Ghosh. (2000) Selective ihibition of NFκB activation by a peptide that blocks the interaction of NEMO with the IκB kinase complex. Science. 289:1550-1554. Moller, D.E. (2000) Potential role of TNF-alpha in the pathogenesis of insulin reisitance and type 2 diabetes. Trends Endocrinol. Metabol. 11:212-217. Moya-Canarena, S.Y., Vanden Heuvel, J.P., Belury, M.A. (1999) Conjugated linoleic acid activates peroxisome proliferator-activated receptor α and β Sparague-Dawley rats. Biochem. Biophys. Acta. 1436:331-335. Navarro, V., Zabala, A., Macarulla, M.T., Fernandez-Quintela, A., Rodringuez, V.M., Simon, E., and Portillo, M.P. (2003) Effects of conjugated linoleic acid on body fat accumulation and serum lipid in hamsters fed an atherogenic diet. J. Physiol. Biochem. 59: 193-198. Neal, J.W., and Clipstone, N.A. (2002) Calcineurin mediates the calcium-dependent inhibition of adipocyte differentiation in 3T3-L1 cells. J. Biol. Chem. 277:49776-81. Nie, M., Corbett, L., Knox, A., and Pang, L. (2005) Differential regulation of chemokine expression by peroxisome proliferator-activated receptor gamma agonists: interactions with glucocorticoids and beta2-agonists J. Biol. Chem. 280: 2550-2561 O’shea, M., Bassaganya-Riera, J., and Mohede, C.M., (2004) Immunomodulatory properties of conjugated linoleic acid. Am. J. Clin.Nutr.79: 1199S-1206S. Okazaki, H., Osuga, J, Tamura, Y., Yahagi, N., Tomita, S., Shionoiri, F. Iizuka, Y., Ohashi, K., Harada, K., Kimura, S., Gotoda, T., Shimano, H., Nobuhiro, Y., and Ishibashi, S. (2002) Lipolysis in the absence of hormone-sensitive lipase. Diabetes 51: 3368-3375. 144
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CHUNG, SOONKYU, Ph. D. Mechanisms b
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MECHANISMS BY WHICH CONJUGATED LINO
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APPROVAL PAGE This Dissertation has
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I also would like to thank The Univ
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IV. PREADIPOCYTES MEDIATE LPS-INDUC
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LIST OF FIGURES ix Page Figure 1.1.
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Figure 4.7. LPS-induced NFκB activ
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in animals and some humans (reviewe
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CLA is potentially effective in: 1)
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Anti-adipogenic Mechanisms of CLA T
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2005). Chapter II of this dissertat
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Figure 1. 2. Multiple mechanisms by
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In contrast, studies conducted in a
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activation. Similarly, Chen et al.
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Central Hypothesis and Specific Obj
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CHAPTER II TRANS-10, CIS-12 CLA INC
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of MEK/ERK signaling by trans-10, c
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Materials and Methods Materials All
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the cultures for additional 12 h. A
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precipitated with ethanol, dried, a
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or for 30 min with 10 µM isoproter
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either 30 µM cis-9, trans-11 CLA o
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dependent effects of CLA on the pho
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Rapamycin Blocks CLA’s Increase i
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Figure 2. 2. Trans-10, cis-12 CLA a
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Figure 2. 6. Trans-10, cis-12 CLA i
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Figure 2. 8. Trans-10, cis-12 CLA-i
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Discussion Feeding mixed CLA isomer
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in lipolysis may occur via an ERK-d
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espectively, by the MEK inhibitor U
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Furthermore, the CLA-mediated incre
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proteins. Inhibition of NFκB activ
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Sopasakis et al. 2004) and insulin
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antibodies for anti-glyceraldehydre
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pM of human insulin in the presence
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were (accession #NM000600) sense (5
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Transfection efficiency was examine
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treatment (Brown et al. 2004), we d
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myotubes (Sinha et al. 2004; Weiger
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controls was found in cytosol (Fig
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Depletion of NFκB p65 by RNAi Atte
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eports of cytokine-mediated insulin
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Figure 3. 2. Trans-10, cis-12 CLA i
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Figure 3. 6. Trans-10, cis-12 CLA-i
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Figure 3. 8. Specific depletion of
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Figure 3. 10. Working model: Trans-
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Based on our working model (Fig 3.1
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mRNA levels of TNF-α (Fig. 3). How
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humans (Riserus et al. 2004a), and
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most likely not impairing the diffe
Page 105 and 106: 1) decreased adipogenic gene expres
Page 107 and 108: adipocytes from WAT in inflammation
Page 109 and 110: differentiation, cultures of SV cel
Page 111 and 112: Immunoblotting and 4MUrea-SDS-PAGE
Page 113 and 114: activity was measured using the Dua
Page 115 and 116: macrophages and myocytes, respectiv
Page 117 and 118: cultures, insulin’s stimulation o
Page 119 and 120: AD90 cultures treated with LPS. Con
Page 121 and 122: Table 4.1. List of human-gene speci
Page 123 and 124: Figure 4.2. Primary cultures of new
Page 125 and 126: Figure 4.4 LPS induction of cytokin
Page 127 and 128: Figure 4. 6. LPS suppresses PPARγ
Page 129 and 130: Figure 4. 8. Inhibitors of NFκB at
Page 131 and 132: Discussion Adipose tissue is a sour
Page 133 and 134: To determine if non-adipocytes othe
Page 135 and 136: attenuation of insulin sensitivity
Page 137 and 138: epression of NFκB target gene expr
Page 139 and 140: EPILOGUE The “Obesity epidemic’
Page 141 and 142: Apart from our initial goal for thi
Page 143 and 144: Concerning membrane specific recept
Page 145 and 146: esearch topic I would like to exami
Page 147 and 148: Berg, A.H., Lin, Y., Lisanti, M.P.,
Page 149 and 150: Chen, C., Koh, A.J., Datta, N.S., Z
Page 151 and 152: Diradourian, C., Girard, J., and Pe
Page 153 and 154: Granlund, L., Juvet, L.K., Pedersen
Page 155: Imamura, M., Inoguchi, T., Ikuyama,
Page 159 and 160: Piper, R.C., Hess, L.J., and James,
Page 161 and 162: Sinha, S., Perdomo, G., Brown, N.F.
Page 163 and 164: Vanden Berghe, W., Vermeulen, L., D
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