- Page 1 and 2: CHUNG, SOONKYU, Ph. D. Mechanisms b
- Page 3: MECHANISMS BY WHICH CONJUGATED LINO
- Page 7 and 8: I also would like to thank The Univ
- Page 9 and 10: IV. PREADIPOCYTES MEDIATE LPS-INDUC
- Page 11 and 12: LIST OF FIGURES ix Page Figure 1.1.
- Page 13 and 14: Figure 4.7. LPS-induced NFκB activ
- Page 15 and 16: in animals and some humans (reviewe
- Page 17 and 18: CLA is potentially effective in: 1)
- Page 19 and 20: Anti-adipogenic Mechanisms of CLA T
- Page 21 and 22: 2005). Chapter II of this dissertat
- Page 23 and 24: Figure 1. 2. Multiple mechanisms by
- Page 25 and 26: In contrast, studies conducted in a
- Page 27 and 28: activation. Similarly, Chen et al.
- Page 29 and 30: Central Hypothesis and Specific Obj
- Page 31 and 32: CHAPTER II TRANS-10, CIS-12 CLA INC
- Page 33 and 34: of MEK/ERK signaling by trans-10, c
- Page 35 and 36: Materials and Methods Materials All
- Page 37 and 38: the cultures for additional 12 h. A
- Page 39 and 40: precipitated with ethanol, dried, a
- Page 41 and 42: or for 30 min with 10 µM isoproter
- Page 43 and 44: either 30 µM cis-9, trans-11 CLA o
- Page 45 and 46: dependent effects of CLA on the pho
- Page 47 and 48: Rapamycin Blocks CLA’s Increase i
- Page 49 and 50: Figure 2. 2. Trans-10, cis-12 CLA a
- Page 51 and 52: Figure 2. 4. Trans-10, cis-12 CLA a
- Page 53 and 54: Figure 2. 6. Trans-10, cis-12 CLA i
- Page 55 and 56:
Figure 2. 8. Trans-10, cis-12 CLA-i
- Page 57 and 58:
Discussion Feeding mixed CLA isomer
- Page 59 and 60:
in lipolysis may occur via an ERK-d
- Page 61 and 62:
espectively, by the MEK inhibitor U
- Page 63 and 64:
Furthermore, the CLA-mediated incre
- Page 65 and 66:
proteins. Inhibition of NFκB activ
- Page 67 and 68:
Sopasakis et al. 2004) and insulin
- Page 69 and 70:
antibodies for anti-glyceraldehydre
- Page 71 and 72:
pM of human insulin in the presence
- Page 73 and 74:
were (accession #NM000600) sense (5
- Page 75 and 76:
Transfection efficiency was examine
- Page 77 and 78:
treatment (Brown et al. 2004), we d
- Page 79 and 80:
myotubes (Sinha et al. 2004; Weiger
- Page 81 and 82:
controls was found in cytosol (Fig
- Page 83 and 84:
Depletion of NFκB p65 by RNAi Atte
- Page 85 and 86:
eports of cytokine-mediated insulin
- Page 87 and 88:
Figure 3. 2. Trans-10, cis-12 CLA i
- Page 89 and 90:
Figure 3. 4. Trans-10, cis-12 CLA a
- Page 91 and 92:
Figure 3. 6. Trans-10, cis-12 CLA-i
- Page 93 and 94:
Figure 3. 8. Specific depletion of
- Page 95 and 96:
Figure 3. 10. Working model: Trans-
- Page 97 and 98:
Based on our working model (Fig 3.1
- Page 99 and 100:
mRNA levels of TNF-α (Fig. 3). How
- Page 101 and 102:
humans (Riserus et al. 2004a), and
- Page 103 and 104:
most likely not impairing the diffe
- Page 105 and 106:
1) decreased adipogenic gene expres
- Page 107 and 108:
adipocytes from WAT in inflammation
- Page 109 and 110:
differentiation, cultures of SV cel
- Page 111 and 112:
Immunoblotting and 4MUrea-SDS-PAGE
- Page 113 and 114:
activity was measured using the Dua
- Page 115 and 116:
macrophages and myocytes, respectiv
- Page 117 and 118:
cultures, insulin’s stimulation o
- Page 119 and 120:
AD90 cultures treated with LPS. Con
- Page 121 and 122:
Table 4.1. List of human-gene speci
- Page 123 and 124:
Figure 4.2. Primary cultures of new
- Page 125 and 126:
Figure 4.4 LPS induction of cytokin
- Page 127 and 128:
Figure 4. 6. LPS suppresses PPARγ
- Page 129 and 130:
Figure 4. 8. Inhibitors of NFκB at
- Page 131 and 132:
Discussion Adipose tissue is a sour
- Page 133 and 134:
To determine if non-adipocytes othe
- Page 135 and 136:
attenuation of insulin sensitivity
- Page 137 and 138:
epression of NFκB target gene expr
- Page 139 and 140:
EPILOGUE The “Obesity epidemic’
- Page 141 and 142:
Apart from our initial goal for thi
- Page 143 and 144:
Concerning membrane specific recept
- Page 145 and 146:
esearch topic I would like to exami
- Page 147 and 148:
Berg, A.H., Lin, Y., Lisanti, M.P.,
- Page 149 and 150:
Chen, C., Koh, A.J., Datta, N.S., Z
- Page 151 and 152:
Diradourian, C., Girard, J., and Pe
- Page 153 and 154:
Granlund, L., Juvet, L.K., Pedersen
- Page 155 and 156:
Imamura, M., Inoguchi, T., Ikuyama,
- Page 157 and 158:
Loscher, C.E., Draper, E., Leavy, O
- Page 159 and 160:
Piper, R.C., Hess, L.J., and James,
- Page 161 and 162:
Sinha, S., Perdomo, G., Brown, N.F.
- Page 163 and 164:
Vanden Berghe, W., Vermeulen, L., D