CHUNG, SOONKYU, Ph. D. Mechanisms by Which Conjugated ...

More documents

Recommendations

Info

Ruan, H., Miles, P.D., Ladd, C.M., Ross, K., Golub, T.R,, Olefsky, J.M,, and Lodish, H.F. (2002) Profiling gene transcription in vivo reveals adipose tissue as an intermediate target for tumor necrosis alpha: implications for insulin resistance. Diabetes 51: 1376-3188 Ruan, H., Pownall, H., and Lodish H (2003) Troglitazone antagonizes tumor necrosis factor-a induced reprogramming of adipocyte gene expression by inhibiting the transcriptional regulatory functions of NFκB. J. Biol. Chem. 278: 28181-28192 Ryder, J.W., Portocarrero, C.P., Song, S.M., and Houseknecht K.L. (2001) Isomerspecific antidiabetic properties of conjugated linoleic acid. Improved glucose tolerance, skeletal muscle insulin action, and UCP-2 gene expression. Diabetes. 50:1149-1157. Saltiel, A.R. (2001) New perspective into the molecular pathogenesis and treatment of type 2 diabetes. Cell 104:517-529. Schmidt, C., Peng, B., Li, Z., Sclabas, G.M., Fujioka, S., Niu, J., Evans, D.B., Abbruzzese, J.L., and Chiao, P.J. (2003) Mechanism of proinflammatory cytokineinduced biphasic NFκB activation. Mol. Cell 12: 1287-1300. Sehat, N., Kramer, J.K., Mossoba, M.M., Yurawecz, M.P., Roach, J.A., Eulitz, K., Morehouse, K.M., and Ku, Y. (1998) Identification of conjugated linoleic acid isomers in cheese by gas chromatography, silver ion high performance liquid chromatography and mass spectral reconstructed ion profiles. Lipids 33:963-971. Serrero, G., Frolov, F., Schroeder, K., Tanaka, L., and Gelhaar, L. (2000) Adipose differentiation related protein: expression, purification of recombinant protein in Escherichia coli and characterization of its fatty acid binding properties. Biochem. Biophys. Acta. 1488: 245-254. Shao, D,, Rangwala, S.M,, Bailey, S.T., Krakow, S.L., Reginato, M.J, Lazar, M.A. (1998) Interdomain communication regulating ligand binding by PPAR-gamma. Nature 396:377-380 Shoelson, S.E., Lee, J., and Yuan, M. (2003) Inflammation and the IKKβ/IκB/NFκB axis in obesity- and diet-induced insulin resistance. Int. J. Obes. 27: 549-552 Shulman, G.I. (2000) Cellular mechanisms of insulin resistance. J. Clin. Invest. 106:171- 176 147
Sinha, S., Perdomo, G., Brown, N.F., and O’Doherty, R.M. (2004) Fatty acid-induced insulin resistance in L6 myotubes is prevented by inhibition of activation and nuclear localization of nuclear factor kappa B. J. Biol. Chem. 297:41294-41301 Skyler, J.S. and Oddo, C. (2002) Diabetes trends in the USA. Diabetes Metab. Res. Rev. 131:S21-26. Smedman, A., Basu, S., Jovinge, S., Fredrikson, G.N., and Vessby, B. (2005) Conjugated linoleic acid increased C-reactive protein in human subjects. Br. J. Nutr. 94:791-795. Soni, K., Lehner, R., Metalnikov, P., O'Donnell, P., Semache, M., Gao, W., Ashman, K., Pshezhetsky, A., and Mitchell, G. (2004) Carboxylesterase 3 (EC 3.1.1.1) is a major adipocyte lipase. J. Biol. Chem. 279: 40683- 40689 Sopasakis, V.R., Sandqvist, M., Gustafson, B., Hammarstedt, A., Schemelz, M., Yang, X., Jansson, P.A., and Smith, U. (2004) High local concentrations and effects on differentiation implicate interleukin-6 as a paracrine regulator. Obesity Res. 12, 454-460 Souza, S., de Vargas, L., Yamamoto, M., Lien, P., Franciosa, M., Moss, L., and Greenberg, A. (1998) Overexpression of perilipin A and B blocks the ability of tumor necrosis factor alpha to increase lipolysis in 3T3-L1 adipocytes. J. Biol. Chem. 273: 24665-24669. Spiegelman, B.M., and Flier, J.S. (2001) Obesity and the regulation of energy balance. Cell 104:531-541. Stuart, P.W., McCann, D., Desai, M., Rosenbaum, M., Leibel, R.L. (2003) Obesity is associated with macrophage accumulation in adipose tissue. J. Clin. Invest. 112:1796- 1808. Suzawa, M., Takada, I., Yanagisawa, J., Ohtake, F., Ogawa, S., Yamauchi, T., Kadowaki, T., Takeudhi, Y., Shibuya, H., Gotoh, Y., Matsumoto, K., and Kato, S. (2003) Cytokines suppress adipogenesis and PPARγ function through the TAK1/TAB1/NIK cascade. Nat. Cell Biol. 5: 224-230 Sztalryd, C., Xu, G., Dorward, H., Tansey, J., Contreras, J., Kimmel, A, and Landos, C. (2003) Perilipin A is essential for the translocation of hormone sensitive lipase during lipolytic activation. J. Cell Biol. 161: 1093-1103 148
Page 1 and 2:
CHUNG, SOONKYU, Ph. D. Mechanisms b
Page 3 and 4:
MECHANISMS BY WHICH CONJUGATED LINO
Page 5 and 6:
APPROVAL PAGE This Dissertation has
Page 7 and 8:
I also would like to thank The Univ
Page 9 and 10:
IV. PREADIPOCYTES MEDIATE LPS-INDUC
Page 11 and 12:
LIST OF FIGURES ix Page Figure 1.1.
Page 13 and 14:
Figure 4.7. LPS-induced NFκB activ
Page 15 and 16:
in animals and some humans (reviewe
Page 17 and 18:
CLA is potentially effective in: 1)
Page 19 and 20:
Anti-adipogenic Mechanisms of CLA T
Page 21 and 22:
2005). Chapter II of this dissertat
Page 23 and 24:
Figure 1. 2. Multiple mechanisms by
Page 25 and 26:
In contrast, studies conducted in a
Page 27 and 28:
activation. Similarly, Chen et al.
Page 29 and 30:
Central Hypothesis and Specific Obj
Page 31 and 32:
CHAPTER II TRANS-10, CIS-12 CLA INC
Page 33 and 34:
of MEK/ERK signaling by trans-10, c
Page 35 and 36:
Materials and Methods Materials All
Page 37 and 38:
the cultures for additional 12 h. A
Page 39 and 40:
precipitated with ethanol, dried, a
Page 41 and 42:
or for 30 min with 10 µM isoproter
Page 43 and 44:
either 30 µM cis-9, trans-11 CLA o
Page 45 and 46:
dependent effects of CLA on the pho
Page 47 and 48:
Rapamycin Blocks CLA’s Increase i
Page 49 and 50:
Figure 2. 2. Trans-10, cis-12 CLA a
Page 51 and 52:
Page 53 and 54:
Figure 2. 6. Trans-10, cis-12 CLA i
Page 55 and 56:
Figure 2. 8. Trans-10, cis-12 CLA-i
Page 57 and 58:
Discussion Feeding mixed CLA isomer
Page 59 and 60:
in lipolysis may occur via an ERK-d
Page 61 and 62:
espectively, by the MEK inhibitor U
Page 63 and 64:
Furthermore, the CLA-mediated incre
Page 65 and 66:
proteins. Inhibition of NFκB activ
Page 67 and 68:
Sopasakis et al. 2004) and insulin
Page 69 and 70:
antibodies for anti-glyceraldehydre
Page 71 and 72:
pM of human insulin in the presence
Page 73 and 74:
were (accession #NM000600) sense (5
Page 75 and 76:
Transfection efficiency was examine
Page 77 and 78:
treatment (Brown et al. 2004), we d
Page 79 and 80:
myotubes (Sinha et al. 2004; Weiger
Page 81 and 82:
controls was found in cytosol (Fig
Page 83 and 84:
Depletion of NFκB p65 by RNAi Atte
Page 85 and 86:
eports of cytokine-mediated insulin
Page 87 and 88:
Figure 3. 2. Trans-10, cis-12 CLA i
Page 89 and 90:
Page 91 and 92:
Figure 3. 6. Trans-10, cis-12 CLA-i
Page 93 and 94:
Figure 3. 8. Specific depletion of
Page 95 and 96:
Figure 3. 10. Working model: Trans-
Page 97 and 98:
Based on our working model (Fig 3.1
Page 99 and 100:
mRNA levels of TNF-α (Fig. 3). How
Page 101 and 102:
humans (Riserus et al. 2004a), and
Page 103 and 104:
most likely not impairing the diffe
Page 105 and 106:
1) decreased adipogenic gene expres
Page 107 and 108:
adipocytes from WAT in inflammation
Page 109 and 110: differentiation, cultures of SV cel
Page 111 and 112: Immunoblotting and 4MUrea-SDS-PAGE
Page 113 and 114: activity was measured using the Dua
Page 115 and 116: macrophages and myocytes, respectiv
Page 117 and 118: cultures, insulin’s stimulation o
Page 119 and 120: AD90 cultures treated with LPS. Con
Page 121 and 122: Table 4.1. List of human-gene speci
Page 123 and 124: Figure 4.2. Primary cultures of new
Page 125 and 126: Figure 4.4 LPS induction of cytokin
Page 127 and 128: Figure 4. 6. LPS suppresses PPARγ
Page 129 and 130: Figure 4. 8. Inhibitors of NFκB at
Page 131 and 132: Discussion Adipose tissue is a sour
Page 133 and 134: To determine if non-adipocytes othe
Page 135 and 136: attenuation of insulin sensitivity
Page 137 and 138: epression of NFκB target gene expr
Page 139 and 140: EPILOGUE The “Obesity epidemic’
Page 141 and 142: Apart from our initial goal for thi
Page 143 and 144: Concerning membrane specific recept
Page 145 and 146: esearch topic I would like to exami
Page 147 and 148: Berg, A.H., Lin, Y., Lisanti, M.P.,
Page 149 and 150: Chen, C., Koh, A.J., Datta, N.S., Z
Page 151 and 152: Diradourian, C., Girard, J., and Pe
Page 153 and 154: Granlund, L., Juvet, L.K., Pedersen
Page 155 and 156: Imamura, M., Inoguchi, T., Ikuyama,
Page 157 and 158: Loscher, C.E., Draper, E., Leavy, O
Page 159: Piper, R.C., Hess, L.J., and James,
Page 163 and 164: Vanden Berghe, W., Vermeulen, L., D
show all

CHUNG, SOONKYU, Ph. D. Mechanisms by Which Conjugated ...

You also want an ePaper? Increase the reach of your titles

Delete template?

Save as template?