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MCI Project Summaries 2008 - Smithsonian Institution

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<strong>MCI</strong> 6206 Morphological Comparison of Alcohol and Formalin Preparation to 3M<br />

Novec Fluids<br />

<strong>MCI</strong> Staff: Mel J. Wachowiak, Paula T. DePriest<br />

The purpose of the analysis was to determine if the Novec fluids provided tissue<br />

preservation that was at least as good as the traditional formalin or alcohol methods.<br />

Preparations of invertebrate specimens tissues (earthworm) were prepared for microtomy,<br />

stained, and documented. All were prepared in the 3M research laboratory. The earthworm<br />

tissues stored in various fluids: formalin, alcohol, and 3M Novec were prepared by microtomy,<br />

stained for light microscopical examination, and documented. Formalin fixed tissue subsequently<br />

stored in Novec was at least as good as alcohol preserved tissue after one year of storage in each<br />

solution.<br />

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