Lecture 7 - Genome Tools

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•http://en.wikipedia.org/wiki/Embryonic_stem_cell •http://en.wikipedia.org/wiki/Stem_cell Stem cells •1960s: EAMcCullock and JETill •Characterized by ability to replicate, renew themselves and differentiating into specialized cells •Two broad types: embryonic (found in blastocyts) and adult stem cells (found in adult tissues) •Pluripotent vs multipotent: multipotent found in adults that form limited number of cell types
•http://en.wikipedia.org/wiki/Embryonic_stem_cell •http://en.wikipedia.org/wiki/Stem_cell Stem cells: “adult stem cells” •Pluripotent vs multipotent: multipotent found in adults that form limited number of cell types •Question of “adult stem cells”- •2005: Pluripotent stem cells from adult fibroblast cultures
Page 1 and 2: Mammals as bioreactor •Use of ani
Page 3 and 4: •http://www.conncoll.edu/ccacad/z
Page 5 and 6: •http://www.chinadaily.com.cn/chi
Page 7 and 8: “…missed it, by this much”
Page 9: •Stem cells
Page 13 and 14: •http://en.wikipedia.org/wiki/Emb
Page 15 and 16: Irving Weissman: hemapoietic stem c
Page 17 and 18: •http://en.wikipedia.org/wiki/Amg
Page 19 and 20: •http://en.wikipedia.org/wiki/Amg
Page 21 and 22: •http://en.wikipedia.org/wiki/Epo
Page 23 and 24: •http://en.wikipedia.org/wiki/Epo
Page 26 and 27: •http://en.wikipedia.org/wiki/Hem
Page 28 and 29: •http://en.wikipedia.org/wiki/Hem
Page 30 and 31: •http://en.wikipedia.org/wiki/Ima
Page 32 and 33: Lecture 6: Chapter 8 •Directed mu
Page 34 and 35: Natural selection and growth: How?
Page 36 and 37: Natural mutation: HBB, beta hemoglo
Page 38 and 39: Another perspective: people and mut
Page 40 and 41: Site-directed mutagenesis •Theore
Page 42 and 43: Tools: deamination repair systems
Page 44 and 45: Enrichment for mutations: dut/ung
Page 46 and 47: Variation: Directed mutagenesis wit
Page 48 and 49: Second generation variation: Random
Page 50 and 51: Random mutagenesis with error-prone
Page 52 and 53: Hybrids generated by shuffling prot
Page 54 and 55: Mutant proteins with non-universal
Page 56 and 57: Protein engineering •2003 •1000
Page 58 and 59: Reasons to mutate: One immediate go
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Example 2: ribonuclease •Bull sem
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Changing Asn to other AAc •At hig
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Increasing enzymatic activity •Mo
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Metal cofactor requirement •Modif
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Decreasing protein sensitivity •S
Page 70 and 71:
Modifying protein specificity •Pr
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Modifying antibodies •Hypervariab
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Modifying two properties: Increasin
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Altering multiple properties simult
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Laundry, detergent and mushrooms
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Molecular analysis of hybrid peroxi
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Therapeutic agents •Horse/cow ser
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Bioprospecting: Microbes with desir
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Aspirin: “Age of Industrializatio
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Natural Products: Tamoxifin •Tamo
Page 91 and 92:
Natural Products: Bioprospecting
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Recombinant proteins for human use
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Hybrid products: INF •IFN cDNA is
Page 97 and 98:
Recombinant modification: hTNF-α
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Optimizing gene expression •Multi
Page 101 and 102:
Treatment with secreting bacteria
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Treatment •Genentech: hDNase I in
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Clearing the lungs 2 with alginate
Page 107 and 108:
Alginate lyase[s] •ORF 69,000 Da
Page 109 and 110:
Phenylketonuria (PKU) •Autosomal
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Lecture 7 - Genome Tools

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