Lecture 7 - Genome Tools

More documents

Recommendations

Info

Enrichment for mutations: dut/ung •Limited by imagination! •Use repair mutants of E. coli •1) dUTPase - (dut) •Elevated dUTP, some incorporated instead of dTTP •2) Uracil N-glycosylase - (ung) •Does not correct out U in DNA •Approx 1% U in place of T •Add oligo plus site-directed mutation •Generate dsDNA as before •Tf to ung + strain •Original strand is “repaired” ie degraded •Mutated strand is sustained
Variation: Directed mutagenesis with plasmid DNA •Drawback to M13-based system •Large number of time-consuming steps to isolated gene •Gets rid of need to subclone gene into M13 vector and subcloning mutated gene back out •Use MCS to clone in •Also, swap out Ap S /Tc R to Ap R /Tc S as selections
Page 1 and 2: Mammals as bioreactor •Use of ani
Page 3 and 4: •http://www.conncoll.edu/ccacad/z
Page 5 and 6: •http://www.chinadaily.com.cn/chi
Page 7 and 8: “…missed it, by this much”
Page 9 and 10: •Stem cells
Page 11 and 12: •http://en.wikipedia.org/wiki/Emb
Page 13 and 14: •http://en.wikipedia.org/wiki/Emb
Page 15 and 16: Irving Weissman: hemapoietic stem c
Page 17 and 18: •http://en.wikipedia.org/wiki/Amg
Page 19 and 20: •http://en.wikipedia.org/wiki/Amg
Page 21 and 22: •http://en.wikipedia.org/wiki/Epo
Page 23 and 24: •http://en.wikipedia.org/wiki/Epo
Page 26 and 27: •http://en.wikipedia.org/wiki/Hem
Page 28 and 29: •http://en.wikipedia.org/wiki/Hem
Page 30 and 31: •http://en.wikipedia.org/wiki/Ima
Page 32 and 33: Lecture 6: Chapter 8 •Directed mu
Page 34 and 35: Natural selection and growth: How?
Page 36 and 37: Natural mutation: HBB, beta hemoglo
Page 38 and 39: Another perspective: people and mut
Page 40 and 41: Site-directed mutagenesis •Theore
Page 42 and 43: Tools: deamination repair systems
Page 46 and 47: Variation: Directed mutagenesis wit
Page 48 and 49: Second generation variation: Random
Page 50 and 51: Random mutagenesis with error-prone
Page 52 and 53: Hybrids generated by shuffling prot
Page 54 and 55: Mutant proteins with non-universal
Page 56 and 57: Protein engineering •2003 •1000
Page 58 and 59: Reasons to mutate: One immediate go
Page 60 and 61: Example 2: ribonuclease •Bull sem
Page 62 and 63: Changing Asn to other AAc •At hig
Page 64 and 65: Increasing enzymatic activity •Mo
Page 66 and 67: Metal cofactor requirement •Modif
Page 68 and 69: Decreasing protein sensitivity •S
Page 70 and 71: Modifying protein specificity •Pr
Page 72 and 73: Modifying antibodies •Hypervariab
Page 74 and 75: Modifying two properties: Increasin
Page 76 and 77: Altering multiple properties simult
Page 78 and 79: Laundry, detergent and mushrooms
Page 80: Molecular analysis of hybrid peroxi
Page 83 and 84: Therapeutic agents •Horse/cow ser
Page 85 and 86: Bioprospecting: Microbes with desir
Page 87 and 88: Aspirin: “Age of Industrializatio
Page 89 and 90: Natural Products: Tamoxifin •Tamo
Page 91 and 92: Natural Products: Bioprospecting
Page 93 and 94: Recombinant proteins for human use
Page 95 and 96:
Hybrid products: INF •IFN cDNA is
Page 97 and 98:
Recombinant modification: hTNF-α
Page 99 and 100:
Optimizing gene expression •Multi
Page 101 and 102:
Treatment with secreting bacteria
Page 103 and 104:
Treatment •Genentech: hDNase I in
Page 105 and 106:
Clearing the lungs 2 with alginate
Page 107 and 108:
Alginate lyase[s] •ORF 69,000 Da
Page 109 and 110:
Phenylketonuria (PKU) •Autosomal
show all

Lecture 7 - Genome Tools

You also want an ePaper? Increase the reach of your titles

Delete template?

Save as template?