Lecture 7 - Genome Tools
Lecture 7 - Genome Tools
Lecture 7 - Genome Tools
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Modifying proteins<br />
•Mimicking natural mutations<br />
•Protocols to change specific amino acids encoded by a cloned gene<br />
•Therapeutic and industrial applications<br />
•Desired results:<br />
•Alter Michaelis constant Km- reflects substrate binding strength<br />
•Maximal rate of conversion Vmax<br />
•[improves efficiency of enzyme-catalyzed reaction Vmax/Km]<br />
•Change thermal tolerance, pH stability<br />
•Modify active site and structure in nonaqueous solvents, non-physiological conditions<br />
•Alter need for co-factor or change co-factor<br />
•Modify substrate-binding site to increase specificity, decrease side reactions<br />
•Link enzymes/proteins together<br />
•Increase resistance to proteases, extending half-life<br />
•Alter allosteric regulation events<br />
•“Addressing” /”export” issues of the protein<br />
•Purification issues<br />
•“Tracking” or visualization of protein