- Page 1 and 2: Mammals as bioreactor •Use of ani
- Page 3 and 4: •http://www.conncoll.edu/ccacad/z
- Page 5 and 6: •http://www.chinadaily.com.cn/chi
- Page 7: “…missed it, by this much”
- Page 11 and 12: •http://en.wikipedia.org/wiki/Emb
- Page 13 and 14: •http://en.wikipedia.org/wiki/Emb
- Page 15 and 16: Irving Weissman: hemapoietic stem c
- Page 17 and 18: •http://en.wikipedia.org/wiki/Amg
- Page 19 and 20: •http://en.wikipedia.org/wiki/Amg
- Page 21 and 22: •http://en.wikipedia.org/wiki/Epo
- Page 23 and 24: •http://en.wikipedia.org/wiki/Epo
- Page 26 and 27: •http://en.wikipedia.org/wiki/Hem
- Page 28 and 29: •http://en.wikipedia.org/wiki/Hem
- Page 30 and 31: •http://en.wikipedia.org/wiki/Ima
- Page 32 and 33: Lecture 6: Chapter 8 •Directed mu
- Page 34 and 35: Natural selection and growth: How?
- Page 36 and 37: Natural mutation: HBB, beta hemoglo
- Page 38 and 39: Another perspective: people and mut
- Page 40 and 41: Site-directed mutagenesis •Theore
- Page 42 and 43: Tools: deamination repair systems
- Page 44 and 45: Enrichment for mutations: dut/ung
- Page 46 and 47: Variation: Directed mutagenesis wit
- Page 48 and 49: Second generation variation: Random
- Page 50 and 51: Random mutagenesis with error-prone
- Page 52 and 53: Hybrids generated by shuffling prot
- Page 54 and 55: Mutant proteins with non-universal
- Page 56 and 57: Protein engineering •2003 •1000
- Page 58 and 59:
Reasons to mutate: One immediate go
- Page 60 and 61:
Example 2: ribonuclease •Bull sem
- Page 62 and 63:
Changing Asn to other AAc •At hig
- Page 64 and 65:
Increasing enzymatic activity •Mo
- Page 66 and 67:
Metal cofactor requirement •Modif
- Page 68 and 69:
Decreasing protein sensitivity •S
- Page 70 and 71:
Modifying protein specificity •Pr
- Page 72 and 73:
Modifying antibodies •Hypervariab
- Page 74 and 75:
Modifying two properties: Increasin
- Page 76 and 77:
Altering multiple properties simult
- Page 78 and 79:
Laundry, detergent and mushrooms
- Page 80:
Molecular analysis of hybrid peroxi
- Page 83 and 84:
Therapeutic agents •Horse/cow ser
- Page 85 and 86:
Bioprospecting: Microbes with desir
- Page 87 and 88:
Aspirin: “Age of Industrializatio
- Page 89 and 90:
Natural Products: Tamoxifin •Tamo
- Page 91 and 92:
Natural Products: Bioprospecting
- Page 93 and 94:
Recombinant proteins for human use
- Page 95 and 96:
Hybrid products: INF •IFN cDNA is
- Page 97 and 98:
Recombinant modification: hTNF-α
- Page 99 and 100:
Optimizing gene expression •Multi
- Page 101 and 102:
Treatment with secreting bacteria
- Page 103 and 104:
Treatment •Genentech: hDNase I in
- Page 105 and 106:
Clearing the lungs 2 with alginate
- Page 107 and 108:
Alginate lyase[s] •ORF 69,000 Da
- Page 109 and 110:
Phenylketonuria (PKU) •Autosomal