Lecture 7 - Genome Tools

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Second generation variation: Random mutagenesis with degenerate oligonucleotide primers •Difficult to know which specific nuc to change •Especially in absence of structural data •Absence of functional data •Mass synthesis of mutants •Screen for ‘best desired’ •Can get unexpected mutants
Random mutagenesis with nucleotide analogs •Nucleotide analog resembles one of four bases •Use repair mechanisms of E. coli to incorporate
Page 1 and 2: Mammals as bioreactor •Use of ani
Page 3 and 4: •http://www.conncoll.edu/ccacad/z
Page 5 and 6: •http://www.chinadaily.com.cn/chi
Page 7 and 8: “…missed it, by this much”
Page 9 and 10: •Stem cells
Page 11 and 12: •http://en.wikipedia.org/wiki/Emb
Page 13 and 14: •http://en.wikipedia.org/wiki/Emb
Page 15 and 16: Irving Weissman: hemapoietic stem c
Page 17 and 18: •http://en.wikipedia.org/wiki/Amg
Page 19 and 20: •http://en.wikipedia.org/wiki/Amg
Page 21 and 22: •http://en.wikipedia.org/wiki/Epo
Page 23 and 24: •http://en.wikipedia.org/wiki/Epo
Page 26 and 27: •http://en.wikipedia.org/wiki/Hem
Page 28 and 29: •http://en.wikipedia.org/wiki/Hem
Page 30 and 31: •http://en.wikipedia.org/wiki/Ima
Page 32 and 33: Lecture 6: Chapter 8 •Directed mu
Page 34 and 35: Natural selection and growth: How?
Page 36 and 37: Natural mutation: HBB, beta hemoglo
Page 38 and 39: Another perspective: people and mut
Page 40 and 41: Site-directed mutagenesis •Theore
Page 42 and 43: Tools: deamination repair systems
Page 44 and 45: Enrichment for mutations: dut/ung
Page 46 and 47: Variation: Directed mutagenesis wit
Page 50 and 51: Random mutagenesis with error-prone
Page 52 and 53: Hybrids generated by shuffling prot
Page 54 and 55: Mutant proteins with non-universal
Page 56 and 57: Protein engineering •2003 •1000
Page 58 and 59: Reasons to mutate: One immediate go
Page 60 and 61: Example 2: ribonuclease •Bull sem
Page 62 and 63: Changing Asn to other AAc •At hig
Page 64 and 65: Increasing enzymatic activity •Mo
Page 66 and 67: Metal cofactor requirement •Modif
Page 68 and 69: Decreasing protein sensitivity •S
Page 70 and 71: Modifying protein specificity •Pr
Page 72 and 73: Modifying antibodies •Hypervariab
Page 74 and 75: Modifying two properties: Increasin
Page 76 and 77: Altering multiple properties simult
Page 78 and 79: Laundry, detergent and mushrooms
Page 80: Molecular analysis of hybrid peroxi
Page 83 and 84: Therapeutic agents •Horse/cow ser
Page 85 and 86: Bioprospecting: Microbes with desir
Page 87 and 88: Aspirin: “Age of Industrializatio
Page 89 and 90: Natural Products: Tamoxifin •Tamo
Page 91 and 92: Natural Products: Bioprospecting
Page 93 and 94: Recombinant proteins for human use
Page 95 and 96: Hybrid products: INF •IFN cDNA is
Page 97 and 98: Recombinant modification: hTNF-α
Page 99 and 100:
Optimizing gene expression •Multi
Page 101 and 102:
Treatment with secreting bacteria
Page 103 and 104:
Treatment •Genentech: hDNase I in
Page 105 and 106:
Clearing the lungs 2 with alginate
Page 107 and 108:
Alginate lyase[s] •ORF 69,000 Da
Page 109 and 110:
Phenylketonuria (PKU) •Autosomal
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Lecture 7 - Genome Tools

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