UltiMate 3000 SRD-3x00 Solvent Racks - Dionex
UltiMate 3000 SRD-3x00 Solvent Racks - Dionex
UltiMate 3000 SRD-3x00 Solvent Racks - Dionex
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<strong>UltiMate</strong> <strong>3000</strong> Series:<br />
<strong>SRD</strong>-<strong>3x00</strong> <strong>Solvent</strong> <strong>Racks</strong><br />
4.3 <strong>Solvent</strong> Reservoirs<br />
All <strong>Solvent</strong> <strong>Racks</strong> are shipped with solvent reservoirs and appropriate tubing, including frit<br />
holders with filter frits.<br />
4.3.1 General Notes<br />
When connecting the solvent reservoirs, observe the following general precautions:<br />
• Observe the precautions for solvent selection (→ page 25).<br />
• Before using the solvent reservoirs for the first time, rinse them thoroughly by using<br />
high-purity solvents.<br />
• Always install filter frits on the solvent supply line filters. This prevents contaminants<br />
from reaching the HPLC system that may increase wear and cause damage to the system.<br />
• Regularly check the filter frits for permeability. This is especially important when using<br />
aqueous solvents. Aqueous solvents may contaminate the filters with algae and other<br />
microorganisms that deposit on the filter frits. Therefore, use fresh solvents at regular<br />
intervals. Clean the reservoirs thoroughly before refilling them and replace the filter frits<br />
as necessary.<br />
• As a standard, filter holders with stainless steel frits are provided in the accessories kit of<br />
the <strong>Solvent</strong> Rack. Do not use these frits if the system includes a biocompatible pump, and<br />
NCS-3500RS or an NCP-3200RS. In this case, replace the stainless steel frits with the<br />
frits from the accessories kit of the module.<br />
Open the filter holder and remove the filter frit. When placing the new filter frit into the<br />
bottom part, make sure that the frit is in a level position (avoid tilting the frit).<br />
• Make sure that the tubing connecting the pump to the degasser is as short as possible and<br />
locate the solvent reservoirs as close as possible to the pump. To avoid formation of air<br />
bubbles in the reservoirs and reformation of air bubbles in the solvent, make sure that the<br />
reservoirs are on the same level or higher as the pump. Therefore, stack the rack onto the<br />
pump as shown in Fig. 4 (→ page 16).<br />
• Normal phase eluents usually show only a low concentration of dissolved gases.<br />
Therefore, it is normally not required to use a degasser with these eluents.<br />
• Before connecting the solvent supply lines, make sure that the connectors are free of<br />
contaminants. Even minute particles can allow air to enter the degasser, and thus reduce<br />
the degassing effectiveness.<br />
Page 20 Operating Instructions