UltiMate 3000 SRD-3x00 Solvent Racks - Dionex
UltiMate 3000 SRD-3x00 Solvent Racks - Dionex
UltiMate 3000 SRD-3x00 Solvent Racks - Dionex
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<strong>UltiMate</strong> <strong>3000</strong> Series:<br />
<strong>SRD</strong>-<strong>3x00</strong> <strong>Solvent</strong> <strong>Racks</strong><br />
Problem Probable Cause Remedial Action<br />
High baseline drift<br />
(Cont'd)<br />
High noise level, nonperiodic<br />
baseline<br />
fluctuation<br />
Periodic baseline<br />
fluctuation, pulsation<br />
Reproducible ghost peaks<br />
in the chromatogram.<br />
Additional peaks appear<br />
in the injection peak.<br />
The eluents are degraded or<br />
inhomogeneous.<br />
The environmental conditions are<br />
unstable.<br />
The mobile phase is delivered in<br />
circles.<br />
For additional causes, refer to the<br />
operating instructions for the<br />
detector.<br />
The eluent is degraded or of poor<br />
quality/purity.<br />
For additional causes, refer to the<br />
operating instructions for the<br />
detector.<br />
There are pressure fluctuations<br />
from the pump.<br />
Before you start an analysis, be<br />
sure that the eluents are already<br />
homogenized in the reservoirs.<br />
Use fresh solvent and check the<br />
eluent filter frits. In aqueous<br />
solvents, growth of<br />
microorganisms is possible.<br />
Make sure that the temperature<br />
and the humidity are constant.<br />
Avoid draft. Verify on the<br />
detector that the lamp and flow<br />
cell covers are in proper<br />
position and that the front panel<br />
door is closed.<br />
Direct the mobile phase to<br />
waste.<br />
→ Detector manual<br />
Use fresh solvents. Use HPLC<br />
grade solvents (MS grade if the<br />
<strong>SRD</strong> is operated together with<br />
an NCS-3500RS or<br />
NCP-3200RS).<br />
→ Detector manual<br />
Purge the pump<br />
(→ Pump manual).<br />
There are air bubbles in the system. Purge the pump<br />
(→ Pump manual).<br />
The degassing channels are<br />
contaminated.<br />
The eluents are degraded, dirty or<br />
of poor purity/quality.<br />
Contamination occurs somewhere<br />
in the system.<br />
With gradients, the equilibration<br />
time after the flush cycle is too<br />
short.<br />
Rinse the degassing channels<br />
(→ page 42).<br />
Use fresh eluent. Use HPLC<br />
grade solvents (MS grade if the<br />
<strong>SRD</strong> is operated together with<br />
an NCS-3500RS or<br />
NCP-3200RS).<br />
Flush the system using an<br />
appropriate solvent.<br />
Extend the equilibration time.<br />
There is excessive dead volume. Eliminate possibly existing<br />
dead volume.<br />
Page 38 Operating Instructions