UltiMate 3000 SRD-3x00 Solvent Racks - Dionex
UltiMate 3000 SRD-3x00 Solvent Racks - Dionex
UltiMate 3000 SRD-3x00 Solvent Racks - Dionex
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<strong>UltiMate</strong> <strong>3000</strong> Series:<br />
<strong>SRD</strong>-<strong>3x00</strong> <strong>Solvent</strong> <strong>Racks</strong><br />
7.2 Rinsing the Degassing Channels<br />
To avoid contamination of the degasser, you should prepare fresh solvents, clean the<br />
solvent supply lines, and rinse the degassing channels at regular intervals.<br />
Rinsing the degassing channels is especially important for the channel that degasses aqueous<br />
solvents (for reverse-phase chromatography).<br />
Usually, it is sufficient to rinse all channels with organic solvent. Use a fresh bottle. When<br />
you use water and acetonitrile or methanol, it is usually sufficient to rinse the degassing<br />
channels once per week.<br />
However, adapt the rinsing intervals to the solvents in use. In persistent cases, for example,<br />
reproducible ghost peaks in the chromatogram, follow these steps:<br />
1. Install a backpressure capillary on the pump outlet. The capillary should be<br />
appropriate for generating a backpressure of 20 to 30 MPa.<br />
2. Rinse the degassing channels for 1 hour with 20% nitric acid at the flow rate normally<br />
used for your application.<br />
3. Rinse the degassing channels with fresh HPLC grade water (MS grade if the <strong>SRD</strong> is<br />
operated together with an NCS-3500RS or NCP-3200RS) until the pH value is neutral.<br />
4. Rinse the degassing channels for two hours with fresh HPLC grade acetonitrile (MS<br />
grade, respectively) at the flow rate normally used for your application.<br />
5. Prepare fresh solvents for your application (using new bottles) and connect them to the<br />
degassing channels.<br />
6. Install new filter frits in the solvent supply line filters.<br />
7. Uninstall the backpressure capillary and reconnect the system as required by your<br />
application.<br />
8. Equilibrate the system.<br />
Page 42 Operating Instructions