PureTec

Processing Product:
a. Note: always close the Buffer and Filtrate valve before starting or stopping the PureTec.
Fill the Diafiltration Vessel with the solution to be processed and close the container.
b. If needed, refill the Exchange Buffer Reservoir and empty the Filtrate Reservoir. Place
the tubing back in both containers.
c. If required, edit the Pump Rate and other parameters entered during the priming process.
Press “Execute” and then “RUN” from the front panel to start the pump. If the system
was not previously primed, a decrease in volume in the Vessel will be observed. This
volume will be recovered at the end of the process.
d. To concentrate the product, keep the Buffer Valve closed and open the Filtrate Valve
and Air Vent Valve. This will reduce the volume in the Diafiltration Vessel as the Filtrate
is removed, thus concentrating the solution. When the desired concentration volume has
been achieved, close the Filtrate and Air Vent Valves.
e. To wash the concentrated solution, open only the Filtrate and Buffer Valves. Once the
solution is concentrated, the PureTec could be stopped, the Cumulative Counters
cleared, and the Filtrate Weight alarm set to stop the pump when the desired amount of
Exchange Buffer has been collected in the Filtrate Reservoir. The balance also allows
you to monitor the Permeate Flow Rate (FP), which can be optimized by altering the
pump rate on the fly from the front panel. Press the “RATE” key, increase or decrease
the rate as needed, and press “C” to select it. The Exchange Buffer will be added at the
same rate the Filtrate is removed by the vacuum created in the Diafiltration Vessel.
If there is more solution to process than the Vessel would originally hold, more can be
added by connecting the Exchange Buffer tubing to a container with additional process
solution. When this container is empty, the pump could be stopped, the tubing
reconnected to the Exchange Buffer, and the solution either concentrated further or
washed.
f. To empty the Diafiltration Vessel at the end of the run, close the Buffer and Filtrate
valves, and Stop the pump. Close the Valve on the Retentate return line at the bottom of
the Vessel. Have a container ready for the transfer. Disconnect the return line at the
valve, and carefully place it in the container. Open the Air Vent valve, and press RUN on
the pump, this will pump the contents of the Diafiltration Vessel out through the pump and
filter cartridge and into the transfer container. Re-connect the tubing, open the Retentate
valve again, and your system is ready for cleaning.
Documentation:
The PureTec displays and prints out several filtration-related parameters. The user can scroll
though three display options providing an instantaneous overview of the filtration progress
and status. All information is printed out in an “Excel-friendly” format. Alternatively, our
SciDoc Software, which uses WinWedge software in conjunction with a customized Excel
spreadsheet, can be used to summarize the filtration data and create graphical
representations of the data on your PC as shown below:
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