mohammad tabish ahmed - eTheses Repository - University of ...

Chapter 3
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Fig 3.13: Images showing complementation of Cpn60.2 from M. smegmatis and M.
tuberculosis in AI90 at 37 deg Celsius. A) Cells plated on LB with 10% sucrose, 100µg/ml
ampicillin, 50µg/ml chloramphenicol, and 0.1 mM IPTG. B) Cells plated on LB with
100µg/ml ampicillin, 50µg/ml chloramphenicol, and 0.1 mM IPTG. C) Cells plated on LB
with 10% sucrose, 100µg/ml ampicillin, and 0.1 mM IPTG.
1- AI90/ pOFESL
2- AI90/ pTrc99a vector
3- AI90/ pTrc-GroES-GroEL
4- AI90/ pTrc-GroEs-Cpn60.2 (M. smegmatis)
5- AI90/ pTrc-GroES-Cpn60.2 (M. tuberculosis)
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