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mohammad tabish ahmed - eTheses Repository - University of ...

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Chapter 4<br />

Dilutions<br />

10 -2 10 -3 10 -4 10 -5 10 -6 10 -7<br />

Dilutions<br />

10 -2 10 -3 10 -4 10 -5 10 -6 10 -7<br />

a1<br />

a2<br />

a3<br />

a4<br />

(a)<br />

b1<br />

b2<br />

b3<br />

b4<br />

b5<br />

(b)<br />

1 2 3 4 5<br />

95 kDa<br />

72 kDa<br />

55 kDa<br />

(c)<br />

Fig 4.10: Complementation and expression <strong>of</strong> JQL and PKR.<br />

Complementation results <strong>of</strong> JQL and PKR in MGM100 at 30°C in the presence <strong>of</strong> 0.2%<br />

arabinose with 0.1 mM IPTG (a) or in 0.2% glucose with 0.1 mM IPTG (b). a1 and b2-<br />

MGM100 pTrc-GroES-PKR, a2 and b1- MGM100 pTrc-GroES-JQL, a3 and b3- MGM100<br />

pTrc-GroES-Cpn60.2, a4 and b4- MGM100 pTrc-GroES-GroEL, b5- MGM100 pTrc99a.<br />

SDS-PAGE showing levels <strong>of</strong> over expression <strong>of</strong> JQL and PKR (c). Samples were taken from<br />

cultures after 4 hours incubation with 0.1 mM IPTG and 0.2% arabinose. Lane 1- Prestained<br />

protein marker (Fermentas), Lane 2- MGM100 pTrc-GroES-JQL; Lane 3: MGM100<br />

pTrc99a, Lane 4: MGM100 pTrc-GroES-PKR, Lane 5: MGM100 pTrc-GroES-GroEL.<br />

170

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