- Page 1: FUNCTIONAL AND STRUCTURAL CHARACTER
- Page 5 and 6: Dedicated to Mum, Dad, and Sharique
- Page 7 and 8: Index of contents Chapter 1: Introd
- Page 9 and 10: 2.4.5: Bacteriophage P1 Transductio
- Page 11 and 12: 4.1.5: Chimeras DHF & GEI 162 4.2:
- Page 13 and 14: List of illustrations Chapter 1: In
- Page 15 and 16: Fig 4.1: Schematic representation o
- Page 17 and 18: List of tables Chapter 1: Introduct
- Page 19 and 20: SDW STPKs TAE TEMED TBS Sterile dis
- Page 21 and 22: Chapter 1 1.1 Protein Folding The g
- Page 23 and 24: Chapter 1 Fig 1.1: The energy lands
- Page 25 and 26: Chapter 1 To try and escape from th
- Page 27 and 28: Chapter 1 Fig 1.2: Pathways regulat
- Page 29 and 30: Chapter 1 As protein folding in Myc
- Page 31 and 32: Chapter 1 1.4 The molecular chapero
- Page 33 and 34: Chapter 1 1.4.1 Classes of molecula
- Page 35 and 36: Chapter 1 Hsp40 DnaJ CbpA DjlA Ydj1
- Page 37 and 38: Chapter 1 obtained. These revertant
- Page 39 and 40: Chapter 1 1.4.3.1 The ribosome asso
- Page 41 and 42: Chapter 1 (A) (B) Fig 1.7: Structur
- Page 43 and 44: Chapter 1 complete the reaction cyc
- Page 45 and 46: Chapter 1 1.4.3.4 The small Hsps On
- Page 47 and 48: Chapter 1 1.4.3.5 Hsp90 family of c
- Page 49 and 50: Chapter 1 Fig 1.11: Structure of Ht
- Page 51 and 52: Chapter 1 1.4.3.6 Hsp100 family of
- Page 53 and 54:
Chapter 1 Fig 1.13: The different r
- Page 55 and 56:
Chapter 1 encoded by more than one
- Page 57 and 58:
Chapter 1 Fig 1.15: The illustratio
- Page 59 and 60:
Chapter 1 (Rommelaere et al., 1993,
- Page 61 and 62:
Chapter 1 Fig 1.16: Octameric struc
- Page 63 and 64:
Chapter 1 main difference between t
- Page 65 and 66:
Chapter 1 1.5.3.3 Prefoldin It has
- Page 67 and 68:
Chapter 1 result the substrate prot
- Page 69 and 70:
Chapter 1 Fig 1.18: The structures
- Page 71 and 72:
Chapter 1 6- If the released peptid
- Page 73 and 74:
Chapter 1 1.5.4.3 GroEL substrates
- Page 75 and 76:
Chapter 1 Recent work has suggested
- Page 77 and 78:
Chapter 1 (A) cpn10 cpn60.1 cpn60.2
- Page 79 and 80:
Chapter 1 1998). Cpn60.1 however ha
- Page 81 and 82:
Chapter 1 1.6 Aims of the project T
- Page 83 and 84:
Chapter 2 Materials and Methods: 2.
- Page 85 and 86:
Chapter 2 pET-cpn10- cpn60.1 Deriva
- Page 87 and 88:
Chapter 2 2.3 Primers: Below is a l
- Page 89 and 90:
Chapter 2 Mut EL.3 Rev groEL 1401-1
- Page 91 and 92:
Chapter 2 29R-EL-60.2 groEL 432-412
- Page 93 and 94:
Chapter 2 Biofilm base media: 13.6g
- Page 95 and 96:
Chapter 2 Preparation of bacterioph
- Page 97 and 98:
Chapter 2 then resuspended in 200µ
- Page 99 and 100:
Chapter 2 - 10X BSA (depending on t
- Page 101 and 102:
Chapter 2 - Colony/culture 1μl - S
- Page 103 and 104:
Chapter 2 The PCR cycling parameter
- Page 105 and 106:
Chapter 2 tubes were placed on ice
- Page 107 and 108:
Chapter 2 2.6 Protein analysis 2.6.
- Page 109 and 110:
Chapter 2 2.6.2- Native gel Reagent
- Page 111 and 112:
Chapter 2 - Diluted primary antibod
- Page 113 and 114:
Chapter 2 supernatant was decanted
- Page 115 and 116:
Chapter 2 2.6.6- Analytical ultrace
- Page 117 and 118:
Chapter 3 Background As discussed a
- Page 119 and 120:
Chapter 3 the -35 region of the trp
- Page 121 and 122:
Chapter 3 20 mins 40 mins 60 mins 8
- Page 123 and 124:
Chapter 3 (a) 60 mins 90 mins 120 m
- Page 125 and 126:
Chapter 3 For this experiment, MGM1
- Page 127 and 128:
Chapter 3 When IPTG was not include
- Page 129 and 130:
Chapter 3 The results from these co
- Page 131 and 132:
Chapter 3 3.2.1 The effect of chang
- Page 133 and 134:
Chapter 3 3.2.2 Using pRARE plasmid
- Page 135 and 136:
Chapter 3 3.3 Complementation and e
- Page 137 and 138:
Chapter 3 of MGM100. The use of Tab
- Page 139 and 140:
Chapter 3 3.3.1 Using pET plasmid i
- Page 141 and 142:
Chapter 3 1 2 3 4 5 6 7 8 95 kDa 72
- Page 143 and 144:
Chapter 3 it only digests the paren
- Page 145 and 146:
Chapter 3 72 kDa 1 2 3 4 5 55 kDa 1
- Page 147 and 148:
Chapter 3 30°C/26°C 37°C MGM100
- Page 149 and 150:
Chapter 3 Fig 3.12: Comparison of t
- Page 151 and 152:
Chapter 3 AI90/pACYC-Ptrc-GroESL-Sa
- Page 153 and 154:
Chapter 3 1 2 3 4 5 6 7 1000 bp 600
- Page 155 and 156:
Chapter 3 10 -1 10 -2 10 -3 10 -4 1
- Page 157 and 158:
Chapter 3 this observation. The res
- Page 159 and 160:
Chapter 3 expression. However, from
- Page 161 and 162:
Chapter 4 Construction and analysis
- Page 163 and 164:
Chapter 4 Background In the last ch
- Page 165 and 166:
Chapter 4 Experimental design To ma
- Page 167 and 168:
Chapter 4 Cpn60.2 7 8 9 Cpn60.1 1 2
- Page 169 and 170:
Chapter 4 The full list of all the
- Page 171 and 172:
Chapter 4 4.1 Imprecise domain swap
- Page 173 and 174:
Chapter 4 4.1.1 Chimera AEC This is
- Page 175 and 176:
Chapter 4 (a) Dilutions 10 -1 10 -2
- Page 177 and 178:
Chapter 4 of GroEL (fig 4.7). This
- Page 179 and 180:
Chapter 4 (a) Dilutions 10 -1 10 -2
- Page 181 and 182:
Chapter 4 4.1.5 Chimeras DHF & GEI
- Page 183 and 184:
Chapter 4 4.2 Precise domain swaps
- Page 185 and 186:
Chapter 4 intermediate domains were
- Page 187 and 188:
Chapter 4 1 2 800 kDa 480 kDa 146 k
- Page 189 and 190:
Chapter 4 Dilutions 10 -2 10 -3 10
- Page 191 and 192:
Chapter 4 is important to note that
- Page 193 and 194:
Chapter 4 Summary the complementati
- Page 195 and 196:
Chapter 4 The other aspect of this
- Page 197 and 198:
Chapter 4 caused due to lack of exp
- Page 199 and 200:
Chapter 4 structures were not expec
- Page 201 and 202:
Chapter 4 Name Construct Blue -Cpn6
- Page 203 and 204:
Chapter 5 Background As discussed i
- Page 205 and 206:
Chapter 5 macromolecules at equilib
- Page 207 and 208:
Chapter 5 5.1 Purification of JNL 5
- Page 209 and 210:
Chapter 5 (a) 1 2 3 4 5 6 7 8 9 10
- Page 211 and 212:
Chapter 5 (a) A B C D E (b) 1 2 3 4
- Page 213 and 214:
Chapter 5 5.2 AUC analysis of JNL U
- Page 215 and 216:
Chapter 5 (a) 1 JNL in Buffer 5 0.9
- Page 217 and 218:
Chapter 5 (a) (b) 100 mM P i 75 mM
- Page 219 and 220:
Chapter 5 In this experiment GroEL
- Page 221 and 222:
Chapter 5 1 2 3 Corrected values Av
- Page 223 and 224:
Chapter 5 expected, evidence of lar
- Page 225 and 226:
Chapter 5 In summary, based on the
- Page 227 and 228:
Chapter 6 In this chapter I will be
- Page 229 and 230:
Chapter 6 of Mscpn60.1 can indirect
- Page 231 and 232:
Chapter 6 can produce products with
- Page 233 and 234:
Chapter 6 As the group in Pittsburg
- Page 235 and 236:
Chapter 6 Fig 6.3: Masses of 4 day
- Page 237 and 238:
Chapter 6 than 7 days, so there is
- Page 239 and 240:
Chapter 6 are required to facilitat
- Page 241 and 242:
Chapter 6 Fig 6.4: Sequence alignme
- Page 243 and 244:
Chapter 6 To ensure that the lack o
- Page 245 and 246:
Chapter 6 6.2.3 Complementation ana
- Page 247 and 248:
Chapter 6 Discussion The double rin
- Page 249 and 250:
Chapter 6 Section 6.3: Testing the
- Page 251 and 252:
Chapter 6 Experimental design For t
- Page 253 and 254:
Chapter 6 EcoRV groEL (1.6kb) HindI
- Page 255 and 256:
Chapter 6 6.3.2 Complementation res
- Page 257 and 258:
Chapter 6 1 2 3 4 72 kDa 55 kDa Fig
- Page 259 and 260:
Chapter 6 10 -1 10 -2 10 -3 10 -4 1
- Page 261 and 262:
Chapter 6 Discussion The results th
- Page 263 and 264:
Summary The main aim of this study
- Page 265 and 266:
together, these results strongly su
- Page 267 and 268:
Basha, E., K. L. Friedrich & E. Vie
- Page 269 and 270:
Cehovin, A., A. R. Coates, Y. Hu, Y
- Page 271 and 272:
Diaz-Acosta, A., M. L. Sandoval, L.
- Page 273 and 274:
Fux, C. A., J. W. Costerton, P. S.
- Page 275 and 276:
Gupta, P., S. Mishra & T. K. Chaudh
- Page 277 and 278:
Hoffmann, A., F. Merz, A. Rutkowska
- Page 279 and 280:
Ito, K., Y. Akiyama, T. Yura & K. S
- Page 281 and 282:
Krzewska, J., G. Konopa & K. Libere
- Page 283 and 284:
Lin, Z., D. Madan & H. S. Rye, (200
- Page 285 and 286:
Mogk, A. & B. Bukau, (2004) Molecul
- Page 287 and 288:
Patzelt, H., G. Kramer, T. Rauch, H
- Page 289 and 290:
Rommelaere, H., M. Van Troys, Y. Ga
- Page 291 and 292:
Strickland, E., B. H. Qu, L. Millen
- Page 293 and 294:
Tyagi, N. K., W. A. Fenton & A. L.
- Page 295:
Yamamori, T. & T. Yura, (1982) Gene