mohammad tabish ahmed - eTheses Repository - University of ...

Chapter 2
Biofilm base media: 13.6g KH 2 PO 4 , 2g (NH 4 ) 2 SO 4 adjust the pH to 7.2 using KOH pellets
then added 0.5mg FeSO 4 .7H 2 O, and 5g Casamino acid.
Biofilm media: 94ml biofilm base media with 5ml of 40% glucose, 1ml 0.1M CaCl 2 , 100μl of
1M MgSO 4 .
2.4.2- Additives
Additive Stock concentration Working concentration
L-Arabinose 20% (w/v) 0.2% (w/v)
D-Glucose 20% (w/v) 0.2% (w/v)
Ampicillin 100mg/ml 100µg/ml
Kanamycin 50mg/ml 50µg/ml
Chloramphenicol 50mg/ml 50µg/ml
Hygromycin 150mg/ml 150μg/ml
IPTG 1 M 1 mM
2.4.3- Growth conditions
E. coli growth conditions
E. coli was grown at 37°C, unless indicated otherwise, in Luria-Bertani (LB) media in a
shaking incubator (180rpm). For overnight cultures, single colonies were picked from a plate
or a loop full of frozen culture and inoculated into 5ml of LB. To subculture, 1ml of the
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