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International Journal of Scientific and Research Publications, Volume 3, Issue 2, February 2013 740<br />
ISSN 2250-3153<br />
Third Author - Anil Mahato, P. G. Center in Chemistry, Smt. S.<br />
M. Panchal Science College, Talod-383215.<br />
Fourth Author - N.B.Mehta, P. G. Center in Chemistry, Smt. S.<br />
M. Panchal Science College, Talod-383215.<br />
www.ijsrp.<strong>org</strong>
International Journal of Scientific and Research Publications, Volume 3, Issue 2, February 2013 739 ISSN 2250-3153 Figure 2: Powder diffraction patterns for the title compound The experimental 2θ range was 2–99° with a step size of 0.01° and a counting time of 60 s per step. The program of graphic tool for powder diffraction named WinPLOTR package 19 was used to determine the observed diffraction peak positions of the title compound. Analytical indexing of the powder pattern and determination of the space group were performed using CRYSFIRE 2002 20 , which is designed to launch the most common indexing programs. Table 1: Crystallographic data for C 21H 26O 5 Unit-cell a 22.4642 (0.0756) Ǻ parameters b 17.1823 (0.0222) Ǻ with c 10.3753(0.0324) Ǻ standard α 90.00 (0.000) ° deviation β 92.93 (1.410) ° γ 90.00(0.000) ° Volume 3999.525 Ǻ 3 Crystal family Monoclinic Space group P 2/m 3.3 Antimicrobial activities Synthesized title compound has been screened for their antimicrobial activity against different panel of <strong>org</strong>anisms, i.e., E.coli, P.aeruginosa, S.aureus, S.pyogenes and antifungal strains C. albicans., using Gentamycin and K. Nystatin as reference standards respectively. The Serial dilution technique was followed by micro method as per NCCLS-1992 manual 21 . The observed Minimum Inhibitory Concentrations (MIC) values, for bacterial and fungal strains of the title compound are presented in Table 2a and Table 2b respectively. Table 2a: Minimal Inhibition Concentrations of Bacterial Strains (MIC) in µg/ml Std. Drug Gentamycin Present study S.aureus MTCC 96 S. pyogenus MTCC 443 E. coli MTCC 442 P. aeruginosa MTCC 441 0.05 1.0 0.25 0.5 6.25 25 100 250 Table 2b: Minimal Inhibition Concentrations of Fungal Strain (MIC) in µg/ml Std. Drug K .Nystatin C. albicans MTCC 227 1000 Present study 500 IV CONCLUSION A novel chalcone derivative, 1-(4-butoxy-2-hydroxyphenyl)-3- (3-bromophenyl) prop-2-en-1-one has been synthesized by chemical method. The product has been confirmed by their chemical analysis, IR, 1H NMR and 13C NMR and further confirms by powder X-ray diffraction study. The antimicrobial activities of the novel chalcone derivative have been investigated and their responses against specific bacteria are good. ACKNOWLEDGMENT The authors are thankful to the management and the principal of Smt. S. M. Panchal Science College, Talod, who provided all kinds of facilities during research work. One of the authors Sahaj Gandhi is also thankful to UGC, New Delhi, the financial support (RFSMS fellowship) to carry out the research work. REFERENCES [1] Nielsen, S.; Mogens, L. F.; Thomas, B.; Kristian, S.; Kromann, H. J. Med. Chem. 2005 48(7), 2667-2677. [2] Somepalli, V.; Marella, P.; Ramchandra S.; Maclabatlula, R.; and Gollumukkala, S. V. Indian J. Chem. 2001 40(B), 495-497. [3] Lopez, S. N.; Castelli, M. V.; Zacchino, S. A.; Dominguez, J. N.; Lobo, G.; Bio<strong>org</strong>. Med. Chem. 2001 9, 1999-2013. [4] Lin, Yuh-Meei; Zhou, Yasheen; Flavin M.T; Zhou, L. M.; Nie, W.; Cheng, F C. Bio <strong>org</strong>. Med. Chem. 2002 10(8), 2795-2802. [5] Paula, L.; Rosana, A.; Maria, A. O.; Susana A.; Piedrafita F.J.; Angel, R. de Lera. J. Med. Chem. 2008 51(17) 5431-5440. [6] Nowakowska, Z. Eur. J. Med. Chem.2007 42, 125–137. [7] Ballesteros J. F.; Sanz M. J., Ubeda A.; Miranda M. A.; Iborra S.; Paya T M.; Alcaraz M. J. J. Med. Chem. 1995 38, 2794-2797. [8] Kamei, R.; Kadokura, M.; Kitagawa, Y.; Hazeki, O.; Oikawa, S. Life Sci., 2003 73, 2091-2099. [9] Tatsuji, E.; Hiromu, O.; Kinuko, N.; Yoko, K.; Katsumi, S.; Masashige, T.; J. Agric. Food Chem. 2007 55, 6013-6017. [10] Christina, R.; Orly, S.; Ute, D.; Nina, S S.; Charles, L. J.; Conrad, K.; J. Med. Chem. 2008 51 (3),659-665. [11] Boeck, P.; Bandeira F.C.; Leal, P.; Yunes, R.; Filho, V.; Torres-Santos, E.; Rossi-Bergmann, B. Bio<strong>org</strong>. Med. Chem. 2006 14, 1538–1545. [12] Liu, M.; Wilairat, P.; Go, M. L. J. Med. Chem. 2001 44, 4443-4452. [13] Chen, M.; Christensen, S. B.; Zhai, L.; Rasmussen, M. H.; Theander, T. G.; Frokjaer, S.; et al. J. Infect. Dis. 1997 176, 1327-1333. [14] Ducki, S.; Forrest, R.; Hadfield, J. A.; Kendall, A.; Lawrence,N. J. et al. Bio<strong>org</strong>. Med. Chem. Lett. 1998 8, 1051-1056. [15] Formanek, K.; Janisch, H.; Kowac, W.; Pharma. Acta. Helv. 1958 33, 437. [16] Parmar, V. S.; Bracke, M. E.; Philippe, J.; Wengel, J.; Jain, S. C.; Olsen, C. E.; Bisht, K. S.; Sharma, Bio<strong>org</strong>. Med. Chem. 1997 5, 1609–1619. [17] Parmar, V. S.; Jain, S. C.; Bisht, K. S.; Sharma H., N. K.; Gupta, S.; Prasad, A. K.; Jha, A.; Poonam et al.; Indian. J. Chem. 1998 37B, 628–643. [18] Patel, U. H.; Gandhi, S. A.; Barot, V. M.; Patel, M. C. Acta Cryst. 2012 E68, o2926-o2927. [19] Roisnel, T. and Rodriguez-Carvajal, J. Mater. Sci. Forum 2001 WinPLOTR: a Windows Tool for Powder Diffraction Patterns Analysis, 118-123. [20] Shirley, R. (2002) The CRYSFIRE 2002 System for Automatic Powder Indexing: User’s Manual, The Lattice Press, Guildford, Surrey, England. User’s Manual, The Lattice Press, Guildford, Surrey, England, 2002. [21] National Committee for Clinical Laboratory Standard. Reference method for broth dilution antifungal susceptibility testing of yeasts Approved standard M27A. (1997), NCCLS, Wayne, PA. AUTHORS First Author - V. M. Barot, P. G. Center in Chemistry, Smt. S. M. Panchal Science College, Talod-383215 Second Author (Correspondence Author) - S. A. Gandhi, Department of Physics, Sardar Patel University, Vallabh Vidyanagar – 388 120. E- mail: sahajg7@gmail.com. www.ijsrp.<strong>org</strong>
International Journal of Scientific and Research Publications, Volume 3, Issue 2, February 2013 740 ISSN 2250-3153 Third Author - Anil Mahato, P. G. Center in Chemistry, Smt. S. M. Panchal Science College, Talod-383215. Fourth Author - N.B.Mehta, P. G. Center in Chemistry, Smt. S. M. Panchal Science College, Talod-383215. www.ijsrp.<strong>org</strong>
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