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International Journal of Scientific and Research Publications, Volume 3, Issue 2, February 2013 830<br />

ISSN 2250-3153<br />

Fig. 6: Effect of Metal on the activity of protease enzyme from AS-3 isolate.<br />

Stability and effect of NaCl<br />

Protease activity was reported at presence and absence of NaCl, without NaCl the activity was highest and decreases with increase in<br />

NaCl concentration from 0 to 5% (Fig. 7). In this study only activity of this is considered in response to NaCl not the production it will<br />

be also worthwhile to check production at varying salt concentrations. Protease activity is found stable at 5% NaCl and 50% retained<br />

upto 4hrs (Fig. 8).<br />

Fig. 7: Activity of crude protease enzyme from AS-3 isolate at various NaCl concentrations.<br />

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