BioProcess Product Guide - GE Healthcare Life Sciences

2
Cell culture
Network of competence speeds your time to
market
As one the world’s leading cell culture companies, PAA
provides expertise in the design, development and
production of culture media. Combining this in-depth
knowledge with the expertise of two business partners,
including cloning and vector design, cell line development,
media optimization, and upstream processing, the expert
team offers its customers individual process optimization
for every aspect of their cell culture requirements.
In addition, PAA can provide support and guidance on
a range of technical issues to make sure its customers
achieve their milestones.
ActiCHO Media System – the all-inclusive
CHO media kit for active cells
PAA has developed a ready-to-use fed-batch and batch
media system for recombinant CHO-DG44 cells. The
ActiCHO Media System for easy feeding and easy scale-up
provides high performance, high protein yield, and speeds
up the process significantly.
The ActiCHO Media System consists of the adaptation
medium ActiCHO SM, the production medium ActiCHO P,
and two matched feeding supplements ActiCHO Feed-A
and ActiCHO Feed-B. The media and supplements do not
contain hydrolysates or animal components, in compliance
with regulatory demands related to virus safety.
The special feature of the ActiCHO Media System is the
fact that PAA offers the system as a ready-to-use starter
kit. After a short adaptation phase, the defined and easy
feeding strategy allows early transfer from shaker flasks
to protein production on a larger scale in a bioreactor. PAA
developed a level 1 starter kit for the initial testing on a
25 mL scale. The next scale up option is a level 2 test kit
with enough material to run a 5 L scale fed-batch. Both
liquid kits are available with or without insulin. Additionally,
PAA offers a 10 L media system starter powder kit. All
kit components can be purchased individually, and
customized production and filling is also available.
Features:
• Ready-to-use starter- and scale-up kit
• Chemically defined
• Significant increase in productivity
• Production of proteins with high potency
• Speeds up time to market
Available ActiCHO Test Kits
ActiCHO Test Kit, Level 1, with or without insulin
Product Volume (mL) Units
ActiCHO SM CD 500 1
ActiCHO P CD 500 1
ActiCHO Feed-A CD 100 2
ActiCHO Feed-B CD 10 2
ActiCHO Test Kit, Level 2, with or without insulin
Product Volume (mL) Units
ActiCHO SM CD 1000 2
ActiCHO P CD 1000 5
ActiCHO Feed-A CD 500 5
ActiCHO Feed-B CD 50 5
ActiCHO Powder Kit – without insulin
Product Prepares for Units
ActiCHO SM Powder Base CD 5 L 1
ActiCHO P Powder Base CD 10 L 1
ActiCHO Feed-A Powder Base CD 5 L 1
ActiCHO Feed-B Powder Base CD 0.5 L 1
For more information about PAA’s products and offerings,
please visit www.gelifesciences.com/paa or www.paa.com
30 www.gelifesciences.com/bioprocess