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Requirements for Unity of Application - Japan Patent Office

Requirements for Unity of Application - Japan Patent Office

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[Example 71]<br />

[Title <strong>of</strong> the Invention]<br />

A process <strong>of</strong> electrochemical analysis and reagent composition<br />

[Claims]<br />

1. A process <strong>of</strong> electrochemical analysis <strong>of</strong> serum iron comprising releasing iron from the<br />

serum sample by adding it to iron-free mixture <strong>of</strong> low-grade fatty alcohol and about<br />

5.5-about 8.5 N HCl, introducing the specified amount <strong>of</strong> this solution into the<br />

electroanalytic vessel, applying the first electrode <strong>for</strong> second ionized iron and copper ion<br />

level detector <strong>of</strong> potential ET2, and in the electroanalytic vessel first ionized iron and copper<br />

ion level detector <strong>of</strong> potential ET1, obtaining the current signal at each electrode, and<br />

comparing them in order to obtain iron level. (See Figure 1)<br />

2. Iron-free reagent to free iron from serum <strong>for</strong> electrochemical analysis <strong>of</strong> serum iron level<br />

comprising low-grade fatty alcohol and about 5.5-about 8.5 N HCl.<br />

[Excerpt from Detail Description <strong>of</strong> the Invention and Drawing]<br />

This invention concerns the process <strong>of</strong> electrochemical analysis <strong>of</strong> iron in blood serum<br />

samples and composition <strong>of</strong> reagent used in this analysis.<br />

In electrochemical analysis <strong>of</strong> serum iron, it is necessary to free iron from serum<br />

composition, and the copper ions present in the solution interferes with iron measurement.<br />

However, when the invention described in Claim 2, the reagent <strong>for</strong> the analysis not only<br />

releases the iron from the serum, enhances the current-voltage curves <strong>of</strong> Fe +2 →Fe +3 +e,<br />

Fe +3 +e→Fe +2 , and shifts the current-voltage curves <strong>of</strong> Cu +2 +e→Cu +1 and separates it from that<br />

<strong>of</strong> Fe +2 →Fe +3 +e, eliminating the confounding effect <strong>of</strong> the copper ions in the solution and<br />

allows an accurate estimate <strong>of</strong> the iron.<br />

Figure 1<br />

[Explanation]<br />

The composition <strong>of</strong> the related invention (Claim 2) does not correspond to “equipment”<br />

but corresponds to “other things” directly used in working the process <strong>of</strong> the electrochemical<br />

analysis <strong>of</strong> the specified invention (Claim 1).<br />

[Concerned Section]<br />

<strong>Patent</strong> Law Section 37(iv)<br />

118

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