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order to achieve and maintain the most<br />

optimized patient care.<br />

377<br />

Measurement of Some Biochemical<br />

Parameters in Serum of Some children<br />

with Autism in Jordan<br />

Abdul Rahman Al-Bazzaz MD*, Rami Al-soufi<br />

* Associated Professor Faculty of Pharmacy<br />

of Al-Ahliyya Amman University. Amman<br />

- Jordan. Associated Professor, Al-Nahrine<br />

University - Faculty of Medicine Department of<br />

physiology and biochemistry. Baghdad -Iraq<br />

albazzazabdo@yahoo.com<br />

Objectives: Is to measure some biochemical<br />

parameters changes like (Homocysteine,<br />

Methylcobalamine (Vit.B12), folic acid,<br />

fasting blood sugar and lipid profiles) in<br />

the serum of autistic children in Jordan<br />

compared with normal subjects matched<br />

with age, sex and body mass index (BMI).<br />

To find if there are any relation between<br />

the blood levels of these parameters and<br />

the cause of autism in Jordan, trying to<br />

give some guide in their treatment. This<br />

is the first study done in Jordan and Arab<br />

countries utilizing all five parameters.<br />

Methods: All the serum biochemical<br />

parameters are measured by special kits<br />

using spectrophotometer, Elisa, and HPLC.<br />

Results: There were significant and<br />

insignificant differences between the<br />

matched patients and controls in the<br />

measured biochemical parameters<br />

Conclusion: A lot of the studies about<br />

autism were done outside Jordan which<br />

specify some other parameters; however,<br />

in this study performed in Jordan we tried<br />

to screen some specifically deficient or<br />

increased serum biochemical parameters<br />

which can have an affect in the diagnosis<br />

and treatment of these children, but until<br />

now Jordan and Arab countries need more<br />

studies.<br />

378<br />

Updated Guidelines in<br />

Immunophenotypic Characterization<br />

of Hematopoietic and Lymphoid<br />

Tumours<br />

Raida Oudat MD, Consultant Hematopathologist<br />

at Hematology Department Princess Iman<br />

Research and Laboratory Sciences Center.<br />

KHMC (Jordan)<br />

droudat@hotmail.com<br />

Objectives: To elaborate and illustrate the<br />

updated guidelines in immunophenotypic<br />

characterization of hematopoietic and<br />

lymphoid tumours using flow cytometry<br />

based on different institutes and societies<br />

with special emphasis on Euro consortium.<br />

Methods: Setting updated<br />

immunophenotypic guidelines for<br />

characterization of hematologic and<br />

lymphoid tumours was based on many<br />

standardization efforts including different<br />

institutes and societies according to<br />

general laboratory practice guidelines.<br />

Results: Standardization guidelines ended<br />

up in the usage of comparable flow<br />

cytometers with full standardization of<br />

instrument settings, laboratory protocols,<br />

immunostaining procedures and careful<br />

selection of antibody panels using 8-color<br />

tubes with the adoption of automated<br />

pattern recognition of normal and<br />

abnormal patient samples.<br />

Conclusion: Applying updated guidelines<br />

in immunophenotypic characterization<br />

of hematopoietic and lymphoid tumours<br />

nowadays is mandatory for definite<br />

diagnosis of difficult and overlapping cases<br />

that cannot be recognized by conventional<br />

flow cytometry.<br />

379<br />

Importance of Infrared Rays and X-ray<br />

Study of the Inhibition of Calcium<br />

Phosphate Lithiasis and Struvite<br />

Crystal Formation using Plant<br />

Extracts<br />

Beghalia Mohamed PhD*, Belouatek Aissa<br />

Ghalem, Said Allali Hocine<br />

University Prof (Algeria)<br />

beghaliamohamed@ymail.com<br />

Objectives: To highlight the importance<br />

of the use of infrared-rays and X-ray<br />

in this work. We performed an in<br />

187 www.jrms.gov.jo

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