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Abstract book 6th RMS 16.indd

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vitro crystallization study enabling the<br />

specification of kinetic and thermodynamic<br />

conditions of formation and growth of<br />

crystalline calcium phosphate<br />

species. We used wild Algerian medicinal<br />

plant extracts which prevent, slow down or<br />

reduce crystallization phases.<br />

Methods: We chose the classical model<br />

for the study of phosphate crystallization<br />

without inhibitor and with it, in order<br />

to assess the inhibiting capacity of any<br />

chemical species used. The precipitation<br />

of the solid phase of phosphates from<br />

artificial urine was studied . The crystal size<br />

development was monitored by polarized<br />

microscopy at different time intervals.<br />

After crystallization time, the mixture was<br />

filtered, the recovered dried precipitates<br />

were analysed by FTIR spectroscopy and<br />

X-rays diffraction technique and chemical<br />

analysis.<br />

Results: In the absence of plant extract<br />

inhibitors (Acacia raddiana, Citrullus<br />

colocynthis, Rhus tripartite), the<br />

crystallization of phosphates led to the<br />

formation of struvite and amorphous<br />

carbonated calcium phosphates (ACCP),<br />

after 6 hours. In presence of lower<br />

concentrations of plant extract inhibitor,<br />

inhibition was partial. The addition of 1 ml<br />

of plant extract to the mixture decreases<br />

the size of crystal. After 4 hours the size<br />

of crystals stabilized at 20.67 μm. The<br />

complete disappearance of struvite crystals<br />

was obtained after addition of 10mL of<br />

plant extract inhibitor. Only Pentahydrated<br />

octocalcium phosphates (POP) and ACCP<br />

were formed. In the presence of other<br />

inhibitor extract plant, the inhibition of<br />

struvite growth and aggregation increased.<br />

The addition of up to a volume of 20 mL<br />

of the second inhibitor resulted in total<br />

inhibition and crystalline transformation<br />

of the ACCP into carbapatite (CA).<br />

Phosphate compounds encountered in<br />

urine can be dangerous and the use of<br />

inhibitors to prevent, slow down or reduce<br />

crystallization phases might be very helpful.<br />

Conclusion: In this investigation, plant<br />

extracts proved to be good inhibitors.<br />

Their effect varies according to solution pH<br />

but they are more efficient in less acidic or<br />

neutral urine than in alkaline one.<br />

Hall E Session 4<br />

Anesthesia<br />

380<br />

Update in Thoracic Anaesthesia<br />

Omar Al-Rawi MD (UK)<br />

Changes in medical technology, patients’<br />

demographics and surgical philosophy have<br />

resulted in older, sicker patients presenting<br />

for thoracic surgery. Simultaneously, a<br />

global economic downturn has mandated<br />

the development of new strategies to<br />

reduce hospital stay. One successful<br />

strategy has been the use of minimally<br />

invasive techniques for a variety of thoracic<br />

surgical procedures. The move to less<br />

invasive surgery and enhanced recovery<br />

has provided thoracic anaesthesia with<br />

the opportunity to evolve to meet these<br />

new challenges. Changes to techniques of<br />

one lung isolation, management of intraoperative<br />

of one lung ventilation and postoperative<br />

analgesia will all be described.<br />

381<br />

Central Neraxial Ultrasound<br />

(Ultrasound of the Spine for Epidural<br />

Placement)<br />

Steven R. Clendenen MD (USA)<br />

Spinal anesthesia and epidural needle<br />

insertion and paravertebral block is usually<br />

a blind technique where the rate of<br />

adverse events depends on the experience<br />

of the operator. Ultrasound can be used to<br />

guide placement of an epidural catheters,<br />

spinal anesthesia and paravertebral block<br />

placement. The ultrasound sonoanatomy<br />

and techniques of cenrtral neuraxial and<br />

paravertebral blocks will be described.<br />

382<br />

Where We’ve Come From & Where<br />

are We Going?<br />

Moawiya Ababneh MD (Jordan)<br />

This lecture will highlight the history<br />

of Anesthesia in Jordan, The Jordan<br />

Medical Council, the Anesthesia Society,<br />

anesthetists Shortage, Current problems<br />

and proposals to solve them.<br />

www.jrms.gov.jo<br />

188

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