Instructions for Use - Glyco Kit MB-LAC Con A - Bruker

Comments
Fast and successful collection of the magnetic beads is dependent on a high quality magnetic
separation device. We strongly recommend the use of a Bruker Magnetic Separator (8-well,
# 65554 or 96-well, # 207151).
Alternatively, elution can be performed with 50 % acetonitrile in 1 % TFA. We strongly recommend
using Acetonitrile hypergrade quality for liquid chromatography. Prepare the solution daily.
: Acetonitrile is highly flammable and harmful (R: 11-20/21/22-36; S: (1/2)-16-36/37), 2% TFA
is irritant (R: 36/38; S: 26-28-37-60)
The beads can also be applied for non-MS approaches. Depending on the subsequent technique
the acidic elution solution may interfere with the following protocol steps. To remove the elution
solution we recommend drying the eluate in a vacuum centrifuge. Afterwards, the dried sample can
be re-dissolved in an appropriate buffer or solution and processed according to the subsequent
protocol. This procedure is also recommended when different glyco-capturing particles are used in
succession.
Different glyco-beads can also be combined in one experiment to capture glyco-proteins with
different binding motifs at once. To do so, the buffer system of either a ConA or LCA kit has to be
used since these buffer systems are compatible with the WGA and/or AIA beads, whereas the
buffers in the WGA and AIA kits are not suitable for the ConA or LCA functionality.
In principle, the amount of beads can be scaled up and down according to the experimental
requirements. When doing this, it is important to keep the ratio of sample volume and ConA-BB
volume constant.
Please note:
After elution using the provided EB, glyco-proteins are denatured. This will have an impact on
folding and functional activity.
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