Instructions for Use - Glyco Kit MB-LAC Con A - Bruker
Instructions for Use - Glyco Kit MB-LAC Con A - Bruker
Instructions for Use - Glyco Kit MB-LAC Con A - Bruker
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Examples<br />
Isolation of a small glycoprotein from a protein mixture<br />
Sample:<br />
Protein mixture consisting of Protein Standard II (1 µl, #207234) and<br />
RNase B (1 µg)<br />
Beads:<br />
<strong>MB</strong>-<strong>LAC</strong> <strong>Con</strong> A (10 µl)<br />
Buffers:<br />
BB 1, WP 1.1, WP 2, EB<br />
Target: MTP AnchorChip 600/384<br />
Matrix:<br />
2,5-DHAP<br />
MALDI mode:<br />
Linear<br />
1500<br />
14920.6<br />
66381.6<br />
Original sample<br />
1000<br />
500<br />
23981.6<br />
44528.4<br />
Intens. [a.u.]<br />
0<br />
1500<br />
1250<br />
1000<br />
750<br />
66399.8<br />
Supernatant<br />
14931.7 23987.7 44548.5<br />
500<br />
250<br />
0<br />
2000<br />
1500<br />
1000<br />
14937.2<br />
Eluate<br />
500<br />
44607.5<br />
0<br />
10000 20000 30000 40000 50000 60000 70000 80000 90000 m/z<br />
RNase was successfully isolated by <strong>MB</strong>-<strong>LAC</strong> <strong>Con</strong> A beads from a protein mixture consisting of<br />
BSA (66 kDa), Protein A (44 kDa) Trypsinogen (23 kDa) and RNase B (14.9 kDa).<br />
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