Instructions for Use - Glyco Kit MB-LAC Con A - Bruker
Instructions for Use - Glyco Kit MB-LAC Con A - Bruker
Instructions for Use - Glyco Kit MB-LAC Con A - Bruker
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Table of <strong>Con</strong>tents<br />
General In<strong>for</strong>mation........................................................................................................................... 2<br />
Intended <strong>Use</strong>..................................................................................................................................... 4<br />
Product description............................................................................................................................ 4<br />
<strong>Kit</strong> Components................................................................................................................................. 5<br />
Storage and lifetime in<strong>for</strong>mation........................................................................................................ 5<br />
Risk and safety in<strong>for</strong>mation ............................................................................................................... 5<br />
General remarks <strong>for</strong> sample handling and MALDI measurements ................................................... 6<br />
Recommended sample preparation procedure ................................................................................. 7<br />
Comments ....................................................................................................................................... 10<br />
MALDI-TOF MS Target Preparation................................................................................................11<br />
Cleaning of MALDI-MS targets.................................................................................................... 11<br />
Cleaning of AnchorChip TM targets ........................................................................................... 11<br />
Cleaning of polished steel targets ........................................................................................... 12<br />
Matrix preparation on the MALDI-MS targets.............................................................................. 13<br />
Analysis of glycoproteins using 2,5-DHAP .............................................................................. 13<br />
Analysis of glycopeptides using 2,5-DHB................................................................................ 14<br />
Analysis of deglycosylated peptides using PAC targets.......................................................... 16<br />
Examples......................................................................................................................................... 17<br />
Isolation of a small glycoprotein from a protein mixture .............................................................. 17<br />
Isolation of glycopetides from tryptic RNase B digest ................................................................. 18<br />
References...................................................................................................................................... 19<br />
Trouble shooting.............................................................................................................................. 20<br />
Ordering In<strong>for</strong>mation ....................................................................................................................... 21<br />
Support............................................................................................................................................ 21<br />
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