Instructions for Use - Glyco Kit MB-LAC Con A - Bruker

8. 1 µl of the turbid suspension has to be
spotted on an AnchorChip TM 600 target
without generating air bubbles.
Spot 1 µl
9. After drying of the spots the target can
be transferred to the MALDI-TOF MS
for measurements.
Dry the spot
Analysis of glycopeptides using 2,5-DHB
For preparation of glycopeptides (e.g. after proteolytic digest of the glycoprotein) within the mass
range of 1 – 10 kDa we recommend the use of AnchorChip 600 targets (#209513) or polished
steel targets (#209520) and 2,5-Dihydroxybenzoic acid (DHB, #201346 or #203074) as matrix.
2,5-DHB preparation on AnchorChip600 targets
1. Dissolve 5 mg DHB in 300 µl
acetonitrile
: DHB is harmful (R: 22-36/37/38;
S: 26-28-36/37-60), acetonitrile is
highly flammable and harmful (R: 11-
20/21/22-36; S: (1/2)-16-36/37)
5 mg DHB
300 µl acetonitrile
2. Add 700 µl TFA (0.1 %) 700 µl 0.1 %TFA
3. Vortex for 1 min at RT Vortex
4. Sonificate for 15 min Sonificate
5. 1 µl of the freshly prepared matrix has to
be spotted onto an AnchorChip 600
target
Spot 1 µl matrix
6. After drying spot 1 µl of sample onto the
matrix
Spot 1 µl sample
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