Instructions for Use - Glyco Kit MB-LAC Con A - Bruker

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Comments Fast and successful collection of the magnetic beads is dependent on a high quality magnetic separation device. We strongly recommend the use of a Bruker Magnetic Separator (8-well, # 65554 or 96-well, # 207151). Alternatively, elution can be performed with 50 % acetonitrile in 1 % TFA. We strongly recommend using Acetonitrile hypergrade quality for liquid chromatography. Prepare the solution daily. : Acetonitrile is highly flammable and harmful (R: 11-20/21/22-36; S: (1/2)-16-36/37), 2% TFA is irritant (R: 36/38; S: 26-28-37-60) The beads can also be applied for non-MS approaches. Depending on the subsequent technique the acidic elution solution may interfere with the following protocol steps. To remove the elution solution we recommend drying the eluate in a vacuum centrifuge. Afterwards, the dried sample can be re-dissolved in an appropriate buffer or solution and processed according to the subsequent protocol. This procedure is also recommended when different glyco-capturing particles are used in succession. Different glyco-beads can also be combined in one experiment to capture glyco-proteins with different binding motifs at once. To do so, the buffer system of either a ConA or LCA kit has to be used since these buffer systems are compatible with the WGA and/or AIA beads, whereas the buffers in the WGA and AIA kits are not suitable for the ConA or LCA functionality. In principle, the amount of beads can be scaled up and down according to the experimental requirements. When doing this, it is important to keep the ratio of sample volume and ConA-BB volume constant. Please note: After elution using the provided EB, glyco-proteins are denatured. This will have an impact on folding and functional activity. Page 10 of 21
MALDI-TOF MS Target Preparation MALDI-TOF MS target preparation is one of the most critical steps for high quality and reproducible results. Depending on the application, different target types and MALDI matrix preparations are favored. For all applications it is mandatory to use a thoroughly cleaned target plate. In dependence on the type of target Bruker Daltonic recommends different cleaning procedures. Cleaning of MALDI-MS targets Cleaning of AnchorChip TM targets This is an advanced cleaning protocol differing from the standard cleaning procedure recommended in the product description. 1. Rinse the target intensively under flowing hot tap water Hot tap water 2. Wipe the target intensively with acetone using a kimwipe kimwipe. : Acetone is highly flammable and irritant (R: 11-36-66-67; S: (2)-9-16-26) Acetone 3. Rinse the target with distilled water (Milli-Q). Distilled water 4. Rinse the target with methanol and let it dry. : Methanol is highly flammable and toxic (R: 11-23/24/25-39/23/24/25; S: (1/2)-7-16-36/37-45) Methanol Page 11 of 21
Page 1 and 2: CARE products are designed for supp
Page 3 and 4: Table of Contents General Informati
Page 5 and 6: Kit Components Materials provided i
Page 7 and 8: Recommended sample preparation proc
Page 9: 14. Transfer the tube from the Magn
Page 13 and 14: Matrix preparation on the MALDI-MS
Page 15 and 16: 7. After drying of the spots the ta
Page 17 and 18: Examples Isolation of a small glyco
Page 19 and 20: References 1 Sparbier K, Koch S, Ke
Page 21: Ordering Information Product Size (

Instructions for Use - Glyco Kit MB-LAC Con A - Bruker

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