Instructions for Use - Glyco Kit MB-LAC Con A - Bruker
Instructions for Use - Glyco Kit MB-LAC Con A - Bruker
Instructions for Use - Glyco Kit MB-LAC Con A - Bruker
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Recommended sample preparation procedure<br />
Important: The sample should not contain any Ca 2+ , Mg 2+ and Mn 2+ chelating agents, e.g. EDTA.<br />
The isolation of glyco-proteins and glyco-peptides requires different buffer systems.<br />
For the isolation of glyco-proteins please use:<br />
BB 1<br />
WB 1.1.<br />
WB 2<br />
EB<br />
For the isolation of glyco-peptides please use:<br />
BB 2<br />
WB 2.1.<br />
WB 2<br />
EB<br />
Binding of samples<br />
1. Re-suspend the Magnetic Beads by shaking<br />
<strong>for</strong> at least 20 times from top to the bottom<br />
and reversed or careful vortexing (do not<br />
sonificate!) to get a homogenous suspension.<br />
Repeat shaking between pipetting steps if<br />
necessary.<br />
Re-suspend the beads<br />
2. Transfer 20 µl of resuspended Magnetic<br />
Beads to a standard thin wall PCR-tube and<br />
add 100 µl WB 1.1 (<strong>for</strong> glyco-proteins) or<br />
WB 2.1 (<strong>for</strong> glyco-peptides).<br />
20 µl beads<br />
100 µl WB1.1 or WB2.1<br />
3. Place the tube in a Magnetic Separator and<br />
move the tubes back and <strong>for</strong>th between<br />
adjacent wells 20 times. Wait <strong>for</strong> 20 seconds<br />
to separate the beads from the supernatant.<br />
Move back and <strong>for</strong>th<br />
Collect beads<br />
4. Remove the supernatant carefully using a<br />
pipette. Avoid contact of pipette tips with the<br />
magnetic beads and take care not to remove<br />
the beads.<br />
Remove supernatant<br />
5. Resuspend the beads in 100 µl WB 1.1 or WB<br />
2.1.<br />
100 µl WB1.1 or WB2.1<br />
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